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当前位置: 首页 > 文章 > 尾叶紫薇开花及花粉萌发研究 浙江农林大学学报 2012,29 (6) 966-970
Position: Home > Articles > Florescence and pollen germination in Lagerstroemia caudate Journal of Zhejiang A&F University 2012,29 (6) 966-970

尾叶紫薇开花及花粉萌发研究

作  者：
王晓玉;徐婉;胡杏;蔡明;潘会堂;张启翔
单  位：
北京林业大学园林学院
关键词：
植物学;尾叶紫薇;开花习性;花粉萌发;花粉储藏
摘  要：
通过观测尾叶紫薇Lagerstroemia caudate花朵的开放过程,并采用离体萌发法测定花粉生活力,研究了尾叶紫薇的开花习性、花粉萌发及储藏条件。结果表明:凌晨3:30尾叶紫薇花萼开裂;6:00花瓣全部展平;6:30花药开始开裂,柱头上开始分泌黏液,进行杂交育种时须于6:30前去雄;6:30-7:30是授粉的最佳时间。采用正交试验方法比较蔗糖、聚乙二醇4000(PEG 4000)、硼酸和氯化钙对尾叶紫薇花粉的离体萌发率的影响。结果表明:最佳离体培养基为100 mg.L-1蔗糖+30 mg.L-1硼酸+30 mg.L-1氯化钙+150 g.L-1PEG 4000,花粉萌发率可达96.28%。尾叶紫薇花粉在自然条件下储藏2 d后生活力急剧下降,6 d后花粉完全失活,4℃是花粉储藏的最佳温度,保存9 d仍能保持30%的萌发率,在散粉后30 d失活。
译  名：
Florescence and pollen germination in Lagerstroemia caudate
作  者：
WANG Xiao-yu1,XU Wan1,HU Xing1,CAI Ming1,2,PAN Hui-tang1,2,ZHANG Qi-xiang1,2(1.College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;2.China National Engineering Research Center for Floriculture,Beijing 100083,China)
关键词：
botany;Lagerstroemia caudate;flowering habit;pollen germination;pollen storage
摘  要：
In order to study the flowering habit and the conditions for pollen germination and storage of Lagerstroemia caudate,the processes of flower opening and pollen viability of Lagerstroemia caudate were observed over a 30 d period.Pollen germination was also tested using orthogonal test of PEG 4000,boric acid,CaCl2,and sucrose.Results showed that at 3:30 sepals of L.caudate started to diverge,at 6:00 petals flattened up,at 6:30 the anther diverged,and at 06:30 the stigma secreted a large amount of mucus.The highest rate of in vitro pollen germination which could reach 96.28% was with sucrose(100 g·L-1) + boric acid(30 mg·L-1) + CaCl2(30 mg·L-1) + PEG 4000(150 g·L-1).Pollen germination in the indoor natural environment decreased sharply from 87% to 65% in 2 d with the pollen losing vigor in 6 d.The best preservation temperature for dry pollen was 4 ℃,in which temperature the dry pollen viability could keep 30%,with dry pollen losing its vigor in 30 d.Thus,as a basis for breeding,if L.caudate was used as a female parent in hybridization,the stamens must be removed before 6:30,and the best pollination time would be at 7:30.

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