Position: Home > Articles > Determination of 20(Fluoro) Quinolones Residues in Livestock and Poultry Meat by TurboFlow Online Cleanup-Liquid Chromatography-Tandem Mass Spectrometry
MEAT RESEARCH
2018
(5)
30-38
TurboFlow在线净化-液相色谱-串联质谱法测定畜禽肉中20种(氟)喹诺酮类兽药残留量
作 者：
王炳玲;田霁昕;项佳林;艾连峰
单 位：
河北出入境检验检疫局;山东农业大学食品科学与工程学院
关键词：
TurboFlow在线净化液相色谱;串联质谱;(氟)喹诺酮类药物;残留;畜禽肉
摘 要：
建立TurboFlow在线净化-液相色谱-串联质谱测定畜禽肉中20种(氟)喹诺酮类药物((Fluoro)quinolones,(F)QNs)残留量的分析方法。样品用甲醇-乙腈(1∶1,V/V)进行均质提取,Cyclone-p在线净化柱净化富集提取液中的(F)QNs,然后将富集所得分析物洗脱,转至Hypersil Gold C_8分析柱,经色谱分离后,用串联质谱检测。结果表明:西诺沙星、伊诺沙星和丹诺沙星在0.2～20.0 ng/mL的线性范围内线性良好,其余17种(F)QNs在0.1～10.0 ng/mL的线性范围内线性良好,且R~2均大于0.99;西诺沙星、伊诺沙星和丹诺沙星的定量限为2μg/kg,其余17种(F)QNs为1μg/kg;20种(F)QNs在3个水平的添加回收率为85.4%～108.2%,相对标准偏差为4.04%～8.57%。方法简单、快速、回收率高、重复性好,适用于动物源食品中(F)QNs的定量及确证检测。
译 名：
Determination of 20(Fluoro) Quinolones Residues in Livestock and Poultry Meat by TurboFlow Online Cleanup-Liquid Chromatography-Tandem Mass Spectrometry
作 者：
WANG Bingling;TIAN Jixin;XIANG Jialin;AI Lianfeng;Hebei Entry-Exit Inspection and Quarantine Bureau;College of Food Science and Engineering, Shandong Agricultural University;
单 位：
WANG Bingling%TIAN Jixin%XIANG Jialin%AI Lianfeng%Hebei Entry-Exit Inspection and Quarantine Bureau%College of Food Science and Engineering, Shandong Agricultural University
关键词：
TurboFlow online cleanup-liquid chromatography;;tandem mass spectrometry;;(fluoro) quinolones;;residue;;livestock and poultry meat
摘 要：
A new method was developed for the determination of(fluoro)quinolones((F)QNs) residues in livestock and poultry meat using TurboFlow online cleanup(TFC)-liquid chromatography-tandem mass spectrometry(LC-MS/MS). Samples were extracted with methanol-acetonitrile(1 : 1, V/V) mixture. Then the extract was purified on a Cyclone-p column. Subsequently, the analytes were separated on a Hypersil Gold C_8 column before being analyzed by tandem mass spectrometry. The calibration curves showed good linearity with R~2 > 0.99 in the concentration range from 0.2 to 20.0 ng/mL for cinoxacin, enrofloxacin and danofloxacin, and in the range from 0.1 to 10.0 ng/mL for 17 other(F)QNs. The limit of quantization for enrofloxacin, danofloxacin and cinoxacin was 2 μg/kg, while that for 17 other(F)QNs was 1 μg/kg. Average recoveries of the analytes fortified at three levels ranged from 85.4% to 108.2%, with relative standard deviations(RSDs) between 4.04% and 8.57%. The method was characterized by simplicity, rapidity, high recovery and good repeatability and was suitable for quantitative analysis and confirmation of(F)QNs residues in foods of animal origin.
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