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当前位置: 首页 > 文章 > 基于高通量测定肉鸡回肠微生物多样性及PICRUSt基因预测分析 动物营养学报 2016,28 (8) 2581-2588
Position: Home > Articles > Analyzing of Ileum Microbial Diversity of Chickens by High-Throughput Sequencing and PICRUSt Predicted Chinese Journal of Animal Nutrition 2016,28 (8) 2581-2588

基于高通量测定肉鸡回肠微生物多样性及PICRUSt基因预测分析

作  者：
徐帅;林奕岑;周梦佳;倪学勤;曾东;曾燕
单  位：
四川农业大学动物医学院动物微生态研究中心
关键词：
肉鸡;回肠;高通量测序;核心菌群;PICRUSt
摘  要：
本试验旨在研究健康肉鸡回肠的微生物群落结构及功能。采用高通量测序技术对15只鸡的回肠内容物进行测序,按照97%的相似度归类操作分类单元(OTUs),去除序列数量少的OTUs(n<2),然后进行生物信息学分析及PICRUSt基因预测。结果表明:1)共获得424 062条tags,并归类于1 002个非单OTUs,样品平均tags为(28 271±8 855)条,平均OTUs为(261±82)个。2)所有样品共含有20个菌门,160个菌属。其中,共享核心菌门4个,分别是厚壁菌门(85.35%)、变形菌门(6.09%)、蓝细菌门(2.15%)和放线菌门(0.16%);共享核心菌属19个,占样品微生物总量的93.75%。3)鸡回肠样品预测得出328个功能。从前20个功能热图可知,各样品的效能存在差异。由此可见,鸡回肠微生物群落复杂,但主要菌群相对稳定。本试验为进一步研究鸡回肠微生物群落结构与功能提供了参考依据。
译  名：
Analyzing of Ileum Microbial Diversity of Chickens by High-Throughput Sequencing and PICRUSt Predicted
作  者：
XU Shuai;LIN Yicen;ZHOU Mengjia;NI Xueqin;ZENG Dong;ZENG Yan;Animal Microecology Institute,College of Veterinary Medicine,Sichuan Agricultural University;
关键词：
chicken;;ileum;;high-throughput sequencing;;core-microbiome;;PICRUSt
摘  要：
The aim of this study was to analyze the composition and function of ileum microbial community of healthy chickens.The samples of ileum contents of 15 chickens were investigated by high-throughput sequencing.Operational taxonomic units(OTUs) were picked with a 97 % similarity threshold and the sequences less than two in all samples were removed.Than the results were analyzed by bioinformation software and PICRUSt functional profiles.The results showed as follows:1) a total of 424 062 tags were assigned to 1 002 non-singleton OTUs from all samples.Each sample had(28 271 ± 8 855) tags and(261 ± 82) OTUs on average.2)In total,20 phyla and 160 genera were detected,and 4 shared core phyla were contained in all samples.They were Firmicutes(85.35 %),Proteobacteria(6.09 %),Cyanobacteria(2.15 %) and Actinobacteria(0.16 %).Nineteen shared core genera were contained in all samples,which consisted 93.75 % of the overall bacteria community.3) Three hundred and twenty-eight functions were detected in all ileum samples.Heatmap of the first 20 functions showed that there were some differences in efficiency of each sample.Therefore,our research reveals that the chicken ileum is complex for the phylogenetic diversity of the microbial communities,but the main microbial communities are relatively stable.The findings of this study will provide basic information and lay the foundation of the research in composition and function of the microbiota of chicken ileum.

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