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当前位置: 首页 > 文章 > 干旱胁迫对转SacB基因、转BADH基因的美丽胡枝子的影响 林业科学 2010,46 (7) 66-70
Position: Home > Articles > Effects of Drought Stress on Lespedeza formosa Plants Expressing SacB Gene & BADH Gene Scientia Silvae Sinicae 2010,46 (7) 66-70

干旱胁迫对转SacB基因、转BADH基因的美丽胡枝子的影响

作  者：
杨晓红;陈晓阳;王颖;周伟;肖铃;宋雅丹
单  位：
北京农学院园林系;华南农业大学林学院
关键词：
美丽胡枝子;转基因植株;干旱胁迫;生理指标
摘  要：
以同时培育的转SacB基因、转BADH基因及未转基因的美丽胡枝子盆栽苗为材料,研究干旱条件下3种试材的脯氨酸、甜菜碱、可溶性糖、过氧化物歧化酶、丙二醛、叶绿素含量的变化。结果表明:在正常土壤水分状态下,3种试材的这几项指标含量没有明显差异,但随着干旱胁迫强度的增加,2类转基因的美丽胡枝子所积累的脯氨酸、可溶性总糖量明显高于非转基因植株,在甜菜碱积累方面,转入BADH基因的美丽胡枝子要强于非转基因植株及转入SacB基因的植株。尽管在干旱状态下,3种试材的SOD活性增强,但2种转基因植株的SOD活性并没有明显大于非转基因植株。不论是转入SacB基因,还是转入BADH基因,转基因植株明显可抑制丙二醛在植物体内的快速积累和叶绿素的降解。田间观测的结果也进一步表明:转基因植株的耐旱性明显强于非转基因植株。
译  名：
Effects of Drought Stress on Lespedeza formosa Plants Expressing SacB Gene & BADH Gene
作  者：
Yang Xiaohong1 Chen Xiaoyang2 Wang Ying1 Zhou Wei1 Xiao Ling1 Song Yadan1 (1. Landscape Department,Beijing University of Agriculture Beijing 102206; 2. College of Forest,Southern China Agricultural University Guangzhou 510642)
关键词：
Lespedeza formosa; transgenic plants; drought stress; physiological indexes
摘  要：
In order to investigate the effects of exogenous SacB gene & BADH gene on Lespedeza formosa under drought stress,potted plants of ordinary and transgenic L. formosa were used as materials. All plants were cultured under the same conditions and then were treated with drought stress. The tolerance to drought was compared among the 3 types of plant materials ( two transgenic and one ordinary ). Proline, betaine, soluble sugar, superoxide dismutase ( SOD ), malonalohyde (MDA) and chlorophyll were measured when the 3 types of plant materials were subjected to drought stress. The results showed that there were no any significant differences in the aforementioned indexes among the 3 material types of plants before drought stress. With the decrease of soil relative water content,the transgenic plants accumulated higher levels of proline and soluble sugar than non-genetically modified control plants. The BADH-transgenic plants accumulated higher level of betaine than SacB-transgenic and control plants. There was no significant difference in SOD activity among 3 material types although they showed higher SOD activity with the increased severity of water deficit. The levels of MDA in plants expressing SacB gene or BADH gene were lower than non-genetically modified plants under drought condition. The levels of chlorophyll in transgenic plants were higher than non-genetically modified plants under water deficit condition. Response of the field plants to natural drought stress further validated that the transgenic L. formosa had higher tolerance to drought stress than non-genetically modified plants.

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