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Position: Home > Articles > Molecular identification of Bursaphelenchus xylophilus by PCR amplification of first internal transcribed spacer of ribosomal DNA Plant Protection 2004,30 (3) 61-63

松材线虫rNDA-ITS1区分子检测与鉴定

作  者:
李一农;余道坚;李芳荣;谢伟宏;陈杰;张青汉
单  位:
深圳出入境检验检疫局
关键词:
植物检疫学;松材线虫;rDNA-ITS1;PCR检测
摘  要:
利用两对松材线虫特异性引物,分别对来自日本、美国和葡萄牙的松材线虫虫样进行PCR检测,成功扩增出330 bp和220 bp rDNA-ITS1区基因片段,该方法对松材线虫成虫或幼虫均能作出准确鉴定。
译  名:
Molecular identification of Bursaphelenchus xylophilus by PCR amplification of first internal transcribed spacer of ribosomal DNA
作  者:
LI Yi-nong, YU Dao-jian, LI Fang-rong, XIE Wei-hong, CHEN Jie, ZHANG Qing-han Shenzhen Entry-Exit Inspection and Quarantine Bureau , Shenzhen 518000, China
关键词:
plant quarantine;Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle;rDNA-ITS1;PCR
摘  要:
DNA fragments of about 220bp and 330bp were amplified by PCR using two pairs of specific primers that were designed from the ribosomal DNA (rNDA). The genomic DNA of Bursaphelenchus xylophilus and Bursaphelenchus mucronatus originated from Japan, United States and Portugal was used as PCR template. This technique can be used to identify the adults or larval of Bursaphelenchus xylophilus exactly.

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