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Position: Home > Articles > Preparation and application of oil emulsion inactivated tetravaccine against ND-IB-EDS-AI Chinese Journal of Veterinary Science 2008,28 (6) 631-633

鸡减蛋性病毒病四联油乳剂灭活疫苗的研制与应用

作  者:
丁伯良;鄢明华;王英珍;李秀丽;张健;侯庆云;王东;张莉
单  位:
天津市畜牧兽医研究所
关键词:
鸡;新城疫;传染性支气管炎;减蛋综合征;H9N2亚型禽流感;四联灭活疫苗
摘  要:
选用鸡新城疫病毒Lasota株、鸡传染性支气管炎M41株、鸡减蛋综合征病毒EDSV76-AV127株及禽流感H9N2亚型Ss株等4株病毒作为制苗用毒种,采用中空纤维超滤浓缩技术对病毒抗原进行浓缩,制成四联油乳剂灭活疫苗。并对其进行了物理性状、无菌检验、安全性、免疫和效力检验及疫苗保存期试验等方面的检验,证明所制备疫苗完全符合质量标准。本疫苗在天津蓟县、宝坻、西青等地进行田间应用试验,共免疫蛋鸡2.8万羽份,结果表明,疫苗免疫保护率达95%。
译  名:
Preparation and application of oil emulsion inactivated tetravaccine against ND-IB-EDS-AI
作  者:
DING BO-liang,YAN Ming-hua,WANG Ying-zhen,LI Xiu-li,ZHANG Jian,HOU Qing-yun,WANG Dong,ZHANG Li(Tianjin Institute of Animal Husbandry and Veterinary Science,Tianjin 300112,China)
关键词:
chicken;ND;IB;EDS;AIV H9N2;inactitated tetravaccine
摘  要:
NDV Lasota,IBV M41,EDS76-AV127 and AIV H9 Ss strains were selected and used to develop and produce oil emulsion inactivated tetravaccine by ultrafiltrate concentrated technique.The prepared inactivated tetravaccine was tested for physical properties,safety,immunity and preservation.Twenty eight thousand poultry layers from Tianjin region were inoculated with the inactivated tetravaccine.The results showed that the protective rate of the inactivated tetravaccines was ninety five percent.

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