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当前位置: 首页 > 文章 > 南亚果实蝇多酚氧化酶的性质研究(英文) 昆虫学报 2011,54 (9) 18-24
Position: Home > Articles > Characterization of polyphenol oxidase from the fruit fly Bactrocera tau(Walker)(Diptera:Tephritidae) Acta Entomologica Sinica 2011,54 (9) 18-24

南亚果实蝇多酚氧化酶的性质研究(英文)

作  者：
李小珍;刘映红
单  位：
西南大学植物保护学院重庆市昆虫学及害虫控制工程重点实验室;江西农业大学农学院
关键词：
南亚果实蝇;发育阶段;多酚氧化酶;酶活性;动力学特性
摘  要：
【目的】为揭示南亚果实蝇Bactrocera tau(Walker)不同发育阶段体内多酚氧化酶的活性与性质。【方法】以邻苯二酚为底物,在415nm波长下测定了南亚果实蝇1,2和3龄幼虫、蛹以及成虫多酚氧化酶的活性和动力学参数。【结果】南亚果实蝇在不同发育阶段,多酚氧化酶的活性存在明显差异,通常3龄幼虫中活性最高,为434.42U/mg;蛹中最低,为231.05U/mg。在pH6.5时,南亚果实蝇不同发育阶段多酚氧化酶的活性分别为265.42,358.34,444.42,210.02和373.99U/mg,但当pH值高于7.0或低于5.0时,多酚氧化酶的活性则明显下降。在温度为34℃和37℃时,南亚果实蝇各发育阶段多酚氧化酶的活性均较高,当温度高于40℃或低于27℃时,活性则明显下降。以邻苯二酚为底物,2龄幼虫中多酚氧化酶的Km值(3.10mmol/min)和Vmax(476.19mmol/L)较大,说明多酚氧化酶对底物邻苯二酚催化能力强;蛹中多酚氧化酶的Km(0.63mmol/min)和Vmax(50.25mmol/L)较小,说明多酚氧化酶对底物的亲和力和催化能力弱。当以L-DOPA为底物时,3龄幼虫中多酚氧化酶的Km值和Vmax较大,分别为0.49mmol/min和188.68mmol/L;蛹中多酚氧化酶的Km值和Vmax较小,分别为0.25mmol/min和21.79mmol/L。【结论】南亚果实蝇体内多酚氧化酶在不同温度和pH值下的活性和动力学参数与虫体发育阶段密切相关。
译  名：
Characterization of polyphenol oxidase from the fruit fly Bactrocera tau(Walker)(Diptera:Tephritidae)
作  者：
LI Xiao-Zhen1,LIU Ying-Hong2,*(1.College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China;2.Chongqing Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400715,China)
关键词：
Bactrocera tau;developmental stage;polyphenol oxidase;enzyme activity;kinetic property
摘  要：
[Objective] This study aims to determine the activities of polyphenoloxidase(PPO) in the fruit fly Bactrocera tau(Walker) during various developmental stages.[Methods] The PPO activity and kinetic properties in the 1st,2nd and 3rd instar larva,pupa and adult were determined with spectrophotomethrical method using catechol as the substrate.[Results] The PPO activities varied significantly during different developmental stages of B.tau.The enzyme activity in the 3rd instar larva was the highest(434.42 U/mg) and that in pupa was the lowest(231.05 U/mg).At pH 6.5,the enzyme activities in the 1st,2nd and 3rd instar larva,pupa and adult were 265.42,358.34,444.42,210.02 and 373.99 U/mg,respectively.However,PPO activities decreased dramatically at pH levels above 7.0 or below 5.0.At 34℃ and 37℃,PPO activities stayed at a high level,while the enzyme activities significantly decreased at above 40℃ or below 27℃.When catechol was used as the substrate,the measured Michaelis-Menten constant(Km) and maximum velocity(Vmax) of PPO in 2nd instar larva were 3.10 mmol/min and 476.19 mmol/L,respectively;but those in pupa were 0.63 mmol/min and 50.25mmol/L,respectively,indicating that the catalytic activity of PPO in 2nd instar larva to the substrate catechol was higher than that in pupa.When L-DOPA was used as the substrate,the measured Km and Vmax of PPO in the 3rd instar larva were 0.49 mmol/min and 188.68 mmol/L,respectively;in contrast,the Km(0.25 mmol/min) and Vmax(21.79 mmol/L) of PPO in pupa were relatively lower.[Conclusion] Our results indicate that the properties of PPO in B.tau at different temperature and pH values are closely associated with its developmental stage.

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