Sexual reproduction and detection of mating-type of Blumeria graminis f.sp.tritici populations

SHI Wen-qi;GONG Shuang-jun;ZENG Fan-song;XUE Min-feng;YANG Li-jun;YU Da-zhao;Institute for Plant Protection and Soil Sciences,Hubei Academy of Agricultural Sciences and Hubei Key Laboratory of Crop Diseases,Insect Pests and Corp Weeds Control;

Blumeria graminis;;wheat;;mating type;;ascomycetes

Pairs of primers were designed according to the partial sequences of MAT1-1-1(GenBank accession No.:HQ171902) MAT1-2-1(GenBank accession No.:HQ171899) genes of Blumeria graminis.f.sp.tritici and screened specific primer sets for detection of the two mating-type genes.Based on PCR amplification,516 of single ascospore isolates established from thirty counties of 10 provinces were employed to detect mating-type genes in this study.The results indicated that the two mating type genes were detected in all of isolates from each of 30 counties,and isolates collected from 28 counties were fit to a ratio of nearly 1:1(MAT1-1-1:MAT1-2-1).16 crosses between isolates of different mating-type were made by pairing randomly and then transferring one isolates to another on plants.Successful formations of cleistothecia after crosses were observed in pairs of the sixteen crosses.Ascospores can release from cleistothecia produced form all of the sixteen crosses.These results in this study provide a basis for further studying role of sexual reproduction in virulence diversity of B.graminis f.sp.tritici.