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Position: Home > Articles > Isolation and Identification of Haemophilus parasuis and Correlative Research China Animal Husbandry & Veterinary Medicine 2014,41 (5) 211-215

副猪嗜血杆菌的分离鉴定及相关特性研究

作  者:
王雪敏;路明华;陈小玲;路迎迎;张培君;龚玉梅;王宏俊
单  位:
北京市农林科学院畜牧兽医研究所;河北工程大学农学院
关键词:
副猪嗜血杆菌;分离鉴定;耐药性;毒力
摘  要:
本试验从2013年北京地区疑似副猪嗜血杆菌病病料中分离到19株革兰氏阴性短小杆菌,对分离株进行培养特性、荚膜染色、生化特性、血清型分型及PCR鉴定,结果显示分离的19株细菌均为副猪嗜血杆菌(Haemophilus parasuis,Hps),分别属于血清4、5、7、12及13型。对分离株进行药敏试验及致病性试验,结果表明各分离株均有多重耐药性,且各分离株除GS-3外均有较强毒力。
译  名:
Isolation and Identification of Haemophilus parasuis and Correlative Research
作  者:
WANG Xue-min;LU Ming-hua;CHEN Xiao-ling;LU Ying-ying;ZHANG Pei-jun;GONG Yu-mei;WANG Hong-jun;College of Agriculture,Hebei University of Engineering;Poultry Diseases Engineering Technology Research Center of Hebei Province;Beijing Municipal Key Laboratory of Animal Disease Prevention and Control Technology,Beijing Institution of Animal Husbandry and Veterinary Medicine,BAAFS;
关键词:
Haemophilus parasuis;;isolation and identification;;drug resistance;;virulence
摘  要:
In 2013,nineteen small gram-negative bacilli were isolated from pigs with suspected infection of Haemophilus parasuis in Beijing.Isolates were identified as Haemophilus parasuis with the culture characteristics,capsule staining,biochemical method,serotype and PCR identification,and these strains belonged to 4,5,7,12,13serotypes.The results of the drug sensitivity test and the pathogenicity test of the nineteen isolates showed that the isolates were multi-drug resistance,and all strains except GS-3had strong toxicity.

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