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Position: Home > Articles > TRANSFORMATION OF POPULUS WITH BG2 GENE Journal of Shandong Agricultural University(Natural Science Edition) 2004,35 (3) 375-378+382

转BG2基因黑杨植株的获得

作  者:
韩琳娜;周春江;崔德才;王斌
单  位:
中国科学院遗传与发育生物学研究所;山东农业大学生命科学学院
关键词:
黑杨;根癌农杆菌;BG2基因;转基因植株
摘  要:
利用根癌农杆菌介导法将 β - 1,3-葡聚糖酶基因 (BG2 )转入黑杨G1。本研究建立了黑杨G1的继代及高频再生系统 ,对传统农杆菌侵染方法进行了改良 ,通过抗生素筛选获得大量抗性植株。对抗性植株进行了PCR和PCR -Southern杂交鉴定 ,证实BG2基因已整合入杨树核基因组中
译  名:
TRANSFORMATION OF POPULUS WITH BG2 GENE
作  者:
HAN Lin-na 1 , ZHOU Chun-jiang 2 , CUI De-cai 1* , WANG Bin 2* (1.College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018,China; 2.Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101,China)
关键词:
Populus,Agrobacterium tumefaciens,Transformation,BG2 gene,Transgenic plants
摘  要:
β-1,3-glucanase ( BG2 )gene was introduced into Populus, G1, mediated by Agrobacterium tumefaciens. The young plants of Populus were used as the material and the subculture system has been established. The traditional transformation method by Agrobacterium tumefaciens was improved and many transgenic plants harbored with BG2 gene have been obtained. PCR and PCR-Southern analysis showed that BG2 gene was integrated into the genome of populus.

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