中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 杨尺蠖核多角体病毒的研究——Ⅳ.病毒多角体的回收试验 林业科学研究 1988 (2) 162-168
Position: Home > Articles > A STUDY ON THE NUCLEAR POLYHEDROSIS VIRUS OF THE POPLAR LOOPER (APOCHEIMA CINERARIUS) Ⅳ. RECOVERY TESTS OF VIRUS Forest Research 1988 (2) 162-168

杨尺蠖核多角体病毒的研究——Ⅳ.病毒多角体的回收试验

作  者：
王贵成;王志贤;崔士英
单  位：
中国林业科学研究院林业研究所
关键词：
杨尺蠖;核多角体病毒;回收方法;工艺流程
摘  要：
本文就杨尺蠖核多角体病毒的几种回收方法进行了比较。回收试验表明,以丙酮—乳糖(7.4—8.4％)共沉法回收 AciNPV 的多角体(PIB)是可行的。制备物的最终含量为 4.96×10~(10)PIB/g,回收率平均达 80.5％。生物活性测定证明,回收的新鲜制备物,对3龄初幼虫的致病力是很好的。其 LC_(50) 及其95％的置信限分别为2.84×10~3PIB/ml和 1.70×10~3—4.96×10~3PIB/ml。 使用该程序,可省去昂贵的离心设备,在实验室一般设备的情况下,一次上样1500—3000 g病死虫,仅需 3小时即可获得最终产品。配制的病毒制剂,可用于防治杨尺蠖 30—65ha。大批量生产证明,该流程操作简便,省工省时,一次上样量较大,完全可以替代离心法,回收的成本也很便宜。
译  名：
A STUDY ON THE NUCLEAR POLYHEDROSIS VIRUS OF THE POPLAR LOOPER (APOCHEIMA CINERARIUS) Ⅳ. RECOVERY TESTS OF VIRUS
作  者：
Wang Guicheng Wang Zhixian Cui Shiying(The Research Institute of Forestry CAF)
关键词：
Apocheima cinerarias; nuclear polyhedrosis virus; recovery tests; recovery procedure
摘  要：
Several methods for recovery procedure of the polyhedral inclusion bodies of nuclear polyhedrosis virus of the poplar looper, Apocheima cinerarius, were compared. Recovery tests indicated that the PIB of AciNPV were precipitated from the concentrated suspensions in 7.4-8.4% solutions of lactose by adding acetone. Counts of the final preparation showed 4.96×1010 PIB/g. Yields of 80.5% were obtained. Bioassay tests indicated that the pathogenicity of the fresh preparations recovered was very good for the early third-instar larvae. The LC50 and 95% fiducial limits acre 2.84×103 PIB/ml and 1.70×103-4.96×103 PIB/ml respectively.In this procedure, costly centrifugal equipment was omitted, 1500-3000g diseased larvae could be reduced to final production in 3hr with laboratory general equipment. In this case, the virus formulation prepared may be used to control the poplar looper larvae in about 30-65 ha in the forest. Mass production has been proved to be a useful replacement for the centrifugation method, and the cost of the recovery virus is cheap.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46