中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Microscopy Observations of Habitable Space in Biochar for Colonization by Fungal Hyphae From Soil 农业科学学报 (英文) 2014,13 (3)
Position: Home > Articles > Microscopy Observations of Habitable Space in Biochar for Colonization by Fungal Hyphae From Soil Journal of Integrative Agriculture 2014,13 (3)

Microscopy Observations of Habitable Space in Biochar for Colonization by Fungal Hyphae From Soil

作  者：
Jaafar, Noraini M.;Clode, Peta L.;Abbott, Lynette K.
单  位：
Univ Western Australia, Sch Earth & Environm, Soil Biol & Mol Ecol Grp, Crawley, WA 6009, Australia;Univ Western Australia, Ctr Microscopy Characterisat & Anal, Crawley, WA 6009, Australia;Univ Putra Malaysia, Fac Agr, Dept Land Management, Serdang 43400, Selangor, Malaysia;Univ Western Australia, UWA Inst Agr, Crawley, WA 6009, Australia
关键词：
biochar;agriculture;fungi;soil;habitable pore space;microscopy
摘  要：
Biochar is a potential micro-environment for soil microorganisms but evidence to support this suggestion is limited. We explored imaging techniques to visualize and quantify fungal colonization of habitable spaces in a biochar made from a woody feedstock. In addition to characterization of the biochar, it was necessary to optimize preparation and observation methodologies for examining fungal colonization of the biochar. Biocliar surfaces and pores were investigated using several microscopy techniques. Biochar particles were compared in soilless media and after deposition in soil. Scanning electron microscopy (SEM) observations and characterization of the biochar demonstrated structural heterogeneity within and among biochar particles. Fungal colonization in and on biochar particles was observed using light, fluorescence and electron microscopy. Fluorescent brightener RR 2200 was more effective than Calcofluor White as a hyphal stain. Biochar retrieved from soil and observed using fluorescence microscopy exhibited distinct hyphal networks on external biochar surfaces. The extent of hyphal colonization of biochar incubated in soil was much less than for biochar artificially inoculated with fungi in a soilless medium. The location of fungal hyphae was more clearly visible using SEM than with fluorescence microscopy. Observations of biochar particles colonized by hyphae from soil posed a range of difficulties including obstruction by the presence of soil particles on biochar surfaces and inside pores. Extensive hyphal colonization of the surface of the biochar in the soilless medium contrasted with limited hyphal colonization of pores within the biochar. Both visualization and quantification of hyphal colonization of surfaces and pores of biochar were restricted by two-dimensional imaging associated with uneven biochar surfaces and variable biochar pore structure. There was very little colonization of biochar from hyphae in the agricultural soil used in this study.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46