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当前位置: 首页 > 文章 > 山豆根多糖对PRRSV感染RAW264.7细胞存活率及分泌炎性因子的影响 南方农业学报 2016,47 (12) 2151-2156
Position: Home > Articles > Effects of Sophora subprostrate polysaccharide on cell viabilities and inflammatory cytokines of RAW264.7 cells infected with porcine reproductive and respiratory syndrome virus(PRRSV) Journal of Southern Agriculture 2016,47 (12) 2151-2156

山豆根多糖对PRRSV感染RAW264.7细胞存活率及分泌炎性因子的影响

作  者：
张玲;李飞;韦英益;何家康;陈海兰;胡庭俊;廖玲玲
单  位：
广西大学动物科学技术学院;广西畜牧研究所
关键词：
猪繁殖与呼吸综合征病毒(PRRSV);山豆根多糖;RAW264.7细胞;存活率;炎性因子
摘  要：
【目的】探讨山豆根多糖(SSP)对猪繁殖与呼吸综合征病毒(PRRSV)体外感染RAW264.7细胞存活率及分泌炎性因子水平的影响,为研发出治疗猪繁殖与呼吸综合征(PRRS)的新型兽药提供参考依据。【方法】以50、100、200和400μg/mL SSP作用于PRRSV体外感染8 h的RAW264.7细胞,通过MTT法评价SSP对PRRSV感染RAW264.7细胞存活率,ELISA检测SSP对PRRSV体外感染RAW264.7细胞培养上清液中的炎性因子TNF-α、IL-1β、IL-6、IL-8、IL-10和MCP-1。【结果】PRRSV感染RAW264.7细胞8 h极显著降低了细胞存活率(P<0.01,下同),而200~400μg/mL SSP能极显著升高PRRSV感染RAW264.7细胞存活率。PRRSV感染RAW264.7细胞8 h可极显著升高细胞分泌TNF-α、IL-1β、IL-6、IL-8、IL-10和MCP-1水平,而SSP能有效降低PRRSV感染RAW264.7细胞分泌上述炎性因子水平,其中以200~400μg/mL SSP的抑制效果最佳。【结论】SSP通过提高炎症细胞存活率及抑制其分泌炎性因子水平,从而有效干预PRRSV感染免疫细胞的炎性反应。
译  名：
Effects of Sophora subprostrate polysaccharide on cell viabilities and inflammatory cytokines of RAW264.7 cells infected with porcine reproductive and respiratory syndrome virus(PRRSV)
作  者：
ZHANG Ling;LI Fei;WEI Ying-yi;HE Jia-kang;CHEN Hai-lan;HU Ting-jun;LIAO Ling-ling;College of Animal Science and Technology,Guangxi University;Guangxi Scientific Experimental Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine;Institute of Apiculture,Chinese Academy of Agricultural Sciences/Key laboratory for Insect-Pollinator Biology,Ministry of Agriculture;Guangxi Institute of Animal Science;Guangxi Kexinyuan Original Breeding Pig Co.,Ltd.;
关键词：
porcine reproductive and respiratory syndrome virus(PRRSV);;Sophora subprostrate polysaccharide;;RAW264.7 cell;;viability;;inflammatory cytokine
摘  要：
【Objective】The present study investigated effects of Sophora suhprostrate polysaccharide(SSP) on cell viabilities and inflammatory cytokines of RAW264.7 cells infected by porcine reproductive and respiratory syndrome virus(PRRSV) in vitro,in order to provide reference for developing new veterinary medicine curing PRRSV.【Method】SSP was applied to RAW264.7 cells infected with PRRSV in vitro for 8 hours at dose of 50,100,200 and 400 μg/mL.Cell viabilities in SSP treated RAW264.7 cells infected by PRRSV in vitro were evaluated by MTT method.ELISA method was used to detect levels of inflammatory cytokines in culture supernatant of RAW264.7 cells,including TNF-α,IL-1β,IL-6,IL-8,IL-10 and MCP-1.【Result】After eight hours of PRRSV infection,viabilities of RAW264.7 cells were significantly reduced(P<0.01,the same below);200-400 μg/mL SSP treatment significantly increased cell viabilities of RAW264.7cells infected by PRRSV.The levels of TNF-a,IL-1β,IL-6,IL-8,IL-10 and MCP-1 were significantly increased in RAW264.7 cells infected with PRRSV.SSP treatment decreased levels of these inflammatory cytokines,and 200-400 μg/mL SSP had the best inhibiting effects.【Conclusion】 SSP can increase cell viabilities and reduce inflammatory cytokine levels in RAW264.7 cells infected by PRRSV,so as to intervene inflammatory response of immune cells infected by PRRSV.

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