中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > SPE结合超高效液相色谱-质谱/质谱仪测定鸡蛋中的万古霉素和去甲万古霉素残留量 食品工业科技 2019 (19) 245-250
Position: Home > Articles > Determination of Vancomycin and Norvancomycin Residue in Egg by SPE with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Science and Technology of Food Industry 2019 (19) 245-250

SPE结合超高效液相色谱-质谱/质谱仪测定鸡蛋中的万古霉素和去甲万古霉素残留量

作  者：
李欣;马宁宁;丁利营;李红艳;康明芹;宋丽丽
单  位：
天津嘉氏堂科技有限公司;长春海关(原吉林检验检疫局);天津中医药大学
关键词：
固相萃取;超高效液相色谱-质谱/质谱;万古霉素;去甲万古霉素;鸡蛋
摘  要：
建立了固相萃取法(SPE)结合超高效液相色谱-质谱/质谱仪(UPLC-MS/MS)测定鸡蛋中万古霉素和去甲万古霉素残留量的方法。样品采用0.1%甲酸水溶液进行提取,Strata-X-C固相萃取柱进行净化,UPLC-MS/MS正离子模式扫描,多反应监测模式(MRM)测定,外标法定量。结果表明:万古霉素和去甲万古霉素在5.0～100.0μg/L浓度范围线性关系良好(r均大于0.999),检出限为2.0μg/kg,定量限为5.0μg/kg,加标平均回收率为85.6%～96.3%,相对标准偏差为3.7%～9.2%。该方法灵敏度高,准确度好,能满足对鸡蛋中万古霉素和去甲万古霉素定量分析的要求。
译  名：
Determination of Vancomycin and Norvancomycin Residue in Egg by SPE with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
作  者：
LI Xin;MA Ning-ning;DING Li-ying;LI Hong-yan;KANG Ming-qin;SONG Li-li;Tianjin University of Traditional Chinese Medicine;Tianjin JiaShiTang Technology Co.,Ltd.;Changchun Customs(Former Jilin Inspection and Quarantine Bureau);
关键词：
solid phase extraction;;UPLC-MS/MS;;vancomycin;;norvancomycin;;egg
摘  要：
The method for the determination of vancomycin and norvancomycin residue in egg was established based on SPE with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). The samples were extracted with mixed solution of 0.1% formic acid,then purified with solid phase extraction(Strata-X-C),determined by UPLC-MS/MS with positive electrospray ionization mode and multiple-reaction monitoring(MRM)and quantified by external standard method. The results showed that the standard calibration curve had good linear behavior in the range from 5.0 to 100.0 μg/L and the relative coefficient was more than 0.999. The limit of detection and quantitation of the method were 2.0 and 5.0 μg/kg,respectively. The spiked recoveries were 85.6%～96.3%,the relative standard deviation was 3.7%～9.2%. The method is sensitive,accurate,and it can meet the demands of quantitative analysis of vancomycin and norvancomycin in egg.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46