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Position: Home > Articles > Reseach of Culture Medium and Cultural Conditions for Antagonistic Marine Mildew TS67 Journal of Henan Agricultural Sciences 2007,36 (6) 72-75

生防菌青霉TS67发酵条件优化研究

作  者:
李海峰;王素英
单  位:
天津商学院
关键词:
生防菌;海洋真菌;培养基;优化;培养条件
摘  要:
研究了发酵条件及培养基组成对新型农用抗生菌海洋真菌TS67发酵的影响,改进后的培养条件为:初始pH 8.0,接种量8%,在180 r/min下28℃振荡培养96 h,优化培养基组成为:葡萄糖5g/L,甘油5 g/L,蛋白胨3 g/L,酵母膏1 g/L,单位发酵液的活性提高了6.6倍;同时表明适量的甘油对活性物质的合成有利。
译  名:
Reseach of Culture Medium and Cultural Conditions for Antagonistic Marine Mildew TS67
作  者:
LI Hai-feng,WANG Su-ying(Tianjin University of Commerce,Tianjin 300134,China)
关键词:
Bio-control microbesi;Marine mildew;Medium;Optimization;Culture condition
摘  要:
The effects of culture medium and cultural conditions on the fermentation of antagonistic marine mildew TS67,a new agricultural antibiotic fungus,were studied.The result showed that nitrogen source presented the most significant effect on the production,and glycerol addition was beneficial.It was concluded that the fermentation activity was increased by 6.6 times to 712u/mL under the optimal conditions as the medium was composed of glucose 5g/L,glycerol 5g/L,peptone 3g/L,yeast extract 1.0g/L,and the optimal cultural conditions was the initial pH value of 8.0,inoculation quantity of 8%,cultural temperature of 28℃,and fermentation period of 96 hours.

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