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牛布氏杆菌病热酚法脂多糖抗原酶联免疫吸附试验(ELISA)的研究

作  者:
张喜悦;王志亮;王君玮;吴延功;徐天刚;徐培莲;赵云玲
单  位:
农业部动物检疫所
关键词:
牛布氏杆菌;ELISA;脂多糖抗原;保存温度
摘  要:
采用世界动物卫生组织(OIE)推荐的热酚法提取的脂多糖(LPS)作为抗原,建立了牛布氏杆菌病的ELISA方法,对45份血清进行检测,证明本ELISA方法灵敏性较高,经阻断试验和交叉试验,证明特异性较好。同时对建立的ELISA方法进行了改进,使制备的牛布氏杆菌ELISA试验试剂盒能在4℃条件下长期保存,具有良好的稳定性和重复性。
译  名:
Studies on ELISA for Detection of Bovine Brucellosis with Heating - phenol Extracted LPS Antigen
作  者:
Zhang XiYue Wang ZhiLiang Wang JunWei Wu YanGong Xu TianGang Xu PeiLian (Animal Quarantine Institute, Qingdao 266032 China)
关键词:
Bovine brucellosis, ELISA, LPS antigen
摘  要:
An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS (lipopolysaccharide) as antigen purified by Heating - phenol recommended by OIE (office international des epizootics) . It indicated the ELBA is sensitive by detecting 45 cattle and specific by blocking test and cross - reacting. Bovine brucellosis ELISA kit was developed based on the study. It was stable at 4℃ for long term, with good repetition.

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