Position: Home > Articles > Multiplex Automated Genome Engineering
Biotechnology Bulletin
2015,31
(6)
60-66
多元自动化基因组工程
作 者：
李丹;高海军
单 位：
北京理工大学生命学院
关键词：
多元自动化基因组工程;基因组编辑技术;基因组工程;大肠杆菌
摘 要：
基因组编辑技术在基因组工程研究中应用广泛,其中位点特异性核酸酶编辑技术和CRISPR/Cas系统在单基因编辑方面贡献卓越,但由于基因组的庞大,这些技术又有一定的局限性。多元自动化基因组工程(MAGE)是一种新型基因组编辑技术,可同时作用于多个基因,具有快速、高效的特点,已被用于大肠杆菌的基因敲除和基因替换。主要介绍了MAGE的原理、具体操作流程及技术进展,并结合MAGE技术的应用,讨论其发展趋势。
译 名：
Multiplex Automated Genome Engineering
作 者：
Li Dan;Gao Haijun;School of Life Sciences,Beijing Institute of Technology;
关键词：
multiplex automated genome engineering;;genome editing;;genome engineering;;Escherichia coli
摘 要：
Genome editing is widely used in genome engineering research and site-specific nuclease technologies and CRISPR/Cas system focus on single gene editing. Owing to the huge size of genome, there are some limitations on the applications of these technologies. Multiplex Automated Genome Engineering(MAGE)is a new, fast and efficient genome editing technology, which can operate multiple genes simultaneously, and be used in knockout and replacement of Escherichia coli genes. This review illustrates the recent advances in the theory, operation protocol and technological innovation of MAGE, its application and development trend were also discussed.
导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
