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屎肠球菌的制备工艺研究

作  者:
徐凯;袁志超;詹泽涛;秦雷;葛宏贺
单  位:
武汉新华扬生物股份有限公司
关键词:
屎肠球菌;喷雾干燥;包衣;海藻糖
摘  要:
试验旨在开发出一套稳定成熟的屎肠球菌制备工艺,以活菌收率为指标,进行离心、喷雾干燥和包衣工艺的优化。试验结果表明,屎肠球菌的最佳离心工艺为离心转速5 000 r·min-1,离心时间10 min;最佳干燥载体为糊精;喷雾干燥的工艺为进风130℃,出风70℃;包衣的包材选择为1%海藻糖。试验通过离心工艺、喷雾干燥工艺和包衣工艺的优化,开发出一套稳定的屎肠球菌制备工艺。
译  名:
Study on the Preparation of Enterococcus Faecium
作  者:
XU Kai;YUAN Zhichao;ZHAN Zetao;QIN Lei;GE Honghe;Wuhan Sunhy Biological Co., Ltd.;
关键词:
Enterococcus faecium;;spray drying;;coating;;trehalose
摘  要:
This study aimed to develop a stable and mature preparation process of Enterococcus faecium. The liv-ing bacterium yield as the index and the test optimizd the spray drying process, centrifugal process and coating pro-cess. The result showed that the best centrifugal process was centrifugation speed 5 000 r·min-1, centrifugation time10 min, the best drying carrier was dextrin. The process of spray drying was as follows: inlet air 130 ℃, outlet air 70 ℃,coating material package 1% trehalose. We could developed a stable excrement enterococcus preparation processthrough the way of optimization the spray drying processcoating and centrifugal process.

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