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当前位置: 首页 > 文章 > 辣椒N基因介导抗根结线虫作用早期表达基因的抑制性消减杂交SSH分析 园艺学报 2007,34 (3) 629-636
Position: Home > Articles > Analysis of Early Expression Genes Resistance to Root Knot Nematode in N Gene Pepper by SSH Acta Horticulturae Sinica 2007,34 (3) 629-636

辣椒N基因介导抗根结线虫作用早期表达基因的抑制性消减杂交SSH分析

作  者：
茆振川;谢丙炎;杨宇红;冯东昕;冯兰香;杨之为
单  位：
西北农林科技大学植物保护学院;中国农业科学院蔬菜花卉研究所
关键词：
根结线虫;辣椒;N基因;抑制性消减杂交;抗线虫相关基因
摘  要：
以含N基因的辣椒(Capsicum annuumL.)为试验材料,接种南方根结线虫(Meloidogyne in-cognita)12、24、36h的根尖材料作为测验方(tester),相应的未接种根尖材料作为驱动方(driver),构建一个南方根结线虫诱导N基因表达早期的正向抑制消减杂交cDNA文库,并结合文库高密度点阵膜杂交差异筛选,获得了237条表达序列标签(EST)。在GenBank上进行BLASTn与BLASTx分析,得到148条功能已知的EST序列,获得已知的上调抗性相关EST68个。分离出了具有NBS-LRR结构的抗线虫蛋白和类LRR抗性蛋白的基因,防御作用相关的类萌芽素(GLP)、HSR203J蛋白、蜜腺蛋白、蛇毒素肽等基因,抗性相关的WRKY、ERFBP等转录因子基因,以及G蛋白、14-3-3蛋白等多种信号蛋白基因。通过Gene Ontology分析,N基因介导的早期表达抗病基因涉及病原物的识别、抗性信号传导、过敏性坏死、系统获得性抗性以及植物细胞保护机制等多个方面,并有许多功能未知的基因有待于进一步的研究。
译  名：
Analysis of Early Expression Genes Resistance to Root Knot Nematode in N Gene Pepper by SSH
作  者：
MAO Zhen-chuan1,2,XIE Bing-yan2,YANG Yu-hong2,FENG Dong-xin2,FENG Lan-xiang2,and YANG Zhi-wei1(1 College of Plant Protection,Northwest A & F University,Yangling,Shaanxi 712100,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
关键词：
Root-knot nematode;Pepper;N gene;Suppression subtractive hybridization(SSH);Resistant-nematode gene
摘  要：
The nematode resistant gene N is a single dominant gene in pepper cultivar Carolina Wonder(Capsicum annuum L.).To investigate the early up-regulated expression genes profile in cultivar Carolina Wonder induced by root-knot nematode(Meloidogyne incognita,RKN),a forward subtracted cDNA library was constructed using suppression subtractive hybridization(SSH).The cDNA of pepper seedling root tips inoculated with J2 RKN for 12,24 and 36 h were used as tester and that from untreated root tips as driver.Totally 237 RKN-induced SSH cDNA fragments were selected with DIG Nonradioactive Nucleic Acid Labeling and Detection System.Based on the sequencing and BLAST analyzing of 148 fragments,68 up-regulated expression EST fragments were identified,including the genes encode nucleotide-binding site/leucine-rich repeat(NBS-LRR) disease resistant proteins and LRR resistance protein-like;some defense proteins,such as Germin-like protein,HSR203J protein-like,Nectarin,and Snakin-2;some biostress resistance related transcription factor like WRKY and ERFBP;some signal proteins,such as GRP-like protein and 14-3-3 family protein.The Gene Ontology analysis revealed that the up-regulated ESTs involved in the recognition to nematode,signal transduction,protection mechanism,HR and SAR.In addition,some ESTs of unknown function may be novel genes.

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