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当前位置: 首页 > 文章 > 秸秆还田后土壤微生物群落结构变化的初步研究 农业环境科学学报 2005,24 (06) 1114-1118
Position: Home > Articles > Changes of Microbial Community Structure in Straw Amended Soil Journal of Agro-Environment Science 2005,24 (06) 1114-1118

秸秆还田后土壤微生物群落结构变化的初步研究

作  者：
赵勇;李武;周志华;张晓君;潘迎捷;赵立平
单  位：
南京农业大学微生物学系;上海交通大学生命科学技术学院微生物分子生态学与基因组学实验室;上海水产大学食品科学学院
关键词：
秸秆;纤维素酶活性;变性梯度凝胶电泳;β-Proteobacteria;纤维素降解菌
摘  要：
通过实验室条件下的小麦秸秆粉和油菜秸秆粉的还土试验,结合常规的分析手段和基于DNA的分子技术(变性梯度凝胶电泳(DGGE)及测序技术),初步研究了秸秆粉还田后土壤物理化学特性及生物学特性的变化。结果表明,培养60d后,秸秆还田土壤的肥力明显提高,其纤维素酶活性明显增强。DGGE图谱表明,对照土壤(S)以及处理土壤(SW和SR),在培养过程中,β-Proteobacteria类细菌组成都在发生变化。对其中一个样品的DGGE条带进行了割胶测序,测序结果表明,大部分条带代表的微生物是未培养的或不可培养的。采用传统分离培养技术,从秸秆还田土壤中分离了2株纤维素降解菌。以上结果说明,秸秆还田具有良好的土壤综合效应。
译  名：
Changes of Microbial Community Structure in Straw Amended Soil
作  者：
ZHAO Yong1,3,LI Wu2,ZHOU Zhi-hua2,ZHANG Xiao-jun2,PAN Ying-jie3,ZHAO Li-ping2(1.Department of Microbiology,Nanjing Agricultural University,Nanjing 210095,China;2.School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China;3.School of Food Science,Shanghai Fisheries University,Shanghai 201106,China)
关键词：
straw;cellulase activity;denaturing gradient gel electrophoresis;β-proteobacteria;cellulolytic bacteria
摘  要：
Changes of physical、 chemical and biological characteristics of soils amended with wheat and rape straws were analyzed by either routine approaches or DNA-based molecular techniques(denaturing gradient gel electrophoresis and sequencing)under laboratory conditions.The results showed that after treatment for 60 days,fertilities of the amended soils increased significantly at range from 23.9% to 52.5% in varying items,but there was little difference for the amended soils for treatment between with wheat and rape straw.Cellulase activities in amended soils were significantly higher than that in the control,and in the first 24 days,the cellulase activities in wheat straw amended soils were higher than that in soils amended with rape straw,in contrast,after 25 days,the case was reversed.DGGE profiles showed that during the incubation period,the composition of β-Proteobacteria in the control and amended soils changed variously.The DGGE bands of one sample were sequenced,and it demonstrated that most of the microorganisms represented by the DGGE bands were uncultured or uncultivable bacteria.Two cellulolytic bacteria were isolated from the straw amended soils by traditional cultivation-dependent technique,one was relative to Cellulosimicrobium cellulans with 99% similarity,and another matched with 98% similarity to Cellulomonas fiavigena.In conclusion,soils amended with straws have beneficial ecological effects.

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