Position: Home > Articles > Study on enzymatic synthesis of phospholipids with conjugated linoleic acid
Science and Technology of Food Industry
2011,32
(7)
189-192
酶法合成共轭亚油酸型磷脂的研究
作 者：
刘丽萍;王卫飞;王永华;杨博;宁正祥
单 位：
华南理工大学轻工与食品学院;华南理工大学生物科学与工程学院
关键词：
共轭亚油酸-磷脂;共轭亚油酸;大豆粉末磷脂;固定化脂肪酶
摘 要：
在非水相体系中利用固定化脂肪酶为催化剂,将共轭亚油酸酰基转移到磷脂分子中,合成富含共轭亚油酸(CLA)的改性磷脂。选用的三种常用的固定化脂肪酶中Lipozyme RM IM催化效率最高,最佳反应条件为:CLA乙酯与大豆粉末磷脂的摩尔比为4∶1,反应温度45℃。对反应产物中的CLA含量进行检测分析表明,48h后其含量可以达到31.3%。进行分离处理后,检测该产品的理化指标。
译 名：
Study on enzymatic synthesis of phospholipids with conjugated linoleic acid
作 者：
LIU Li-ping1,WANG Wei-fei1,WANG Yong-hua1,,YANG Bo2,NING Zheng-xiang1 (1.College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China;2.College of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510640,China)
关键词：
CLA-PL;CLA;soybean phospholipids;immobilized lipase
摘 要：
In non-aqueous system,using immobilized lipase as catalyst,conjugated linoleic acid-phospholipids were produced by transferring CLA-ethyl ester acyl to phospholipids.Screening of three lipases showed that Lipozyme RM IM was most effective in incorporation of CLA into phospholipids(PL),and the processing parameters were optimized as follows:molar ratio of CLA-ethyl ester to soybean phospholipids 4∶1,reaction temperature 45℃.After 48h,the content of CLA in the products was 31.3%.Through separation treatment,physical and chemical index of products were detected.
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