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当前位置: 首页 > 文章 > Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars 农业科学学报 (英文) 2019,18 (12)
Position: Home > Articles > Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars Journal of Integrative Agriculture 2019,18 (12)

Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars

作  者：
Meng Lin;Dong Jian-xin;Wang Shu-sheng;Song Ke;Ling Ai-fen;Yang Jin-guang;Xiao Zhi-xin;Li Wei;Song Wen-jing;Liang Hong-bo
单  位：
Hongyunhonghe Tobacco Grp Co Ltd, Kunming 650231, Yunnan, Peoples R China;Sichuan Tobacco Corp, Liangshan Branch, Xichang 615000, Peoples R China;Yunnan Tobacco Co, Baoshan Branch, Baoshan 678000, Peoples R China;Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Tobacco Biol & Proc, Tobacco Res Inst, Qingdao 266101, Shandong, Peoples R China
关键词：
auxin;lateral root;formation and elongation;tobacco;NH4+/NO3- ratio
摘  要：
Nitrogen (N), the major forms of which are nitrate (NO3-) and ammonium (NH4+), plays an important role in plant growth and mediation of root development. However, the role of auxin in root growth in response to different NH4+/NO3- ratios remains unclear. Two tobacco cultivars (Nicotiana tabacum L.) were adopted in this study, which displayed variant growth features under the situations with sole NO3- nutrition ratio (NH4+/NO3- ratio: 0/100), low NO3- nutrition ratio (NH4+/NO3- ratio: 97/3), and optimal NH4+/NO3- ratio (50/50). We investigated the effects of the different NH4+/NO3- ratios on the formation and elongation of lateral roots (LRs), auxin concentration, DR5::GUS expression, H-3-labeled indole acetic acid ([H-3]IAA) transport, and the expression of six PIN genes in tobacco roots. We also examined the effects of exogenous auxin and a transport inhibitor on LRs growth. The results are shown as follows, compared to optimal N nutrition conditions, the biomass and nitrogen (N) accumulation were largely reduced by sole and low NO3- nutrition treatment in NC89, but no difference was observed in Zhongyan 100. In most cases, sole and low NO3- nutrition impaired the elongation and formation of first-order lateral roots (1 degrees LRs), only in NC89, thus reducing the root growth. IAA concentration and DR5::GUS expression levels decreased in roots when NC89 was subjected to sole and low NO3- nutrition media, suggesting that different NH4+/NO3- ratios affect the transport of auxin from leaves to roots. Results were similar following exogenous NAA application to low NO3- nutrition treated seedlings. Based on direct [H-3]IAA transport measurement, the transport of polar auxin from shoots to roots decreased due to low NO3- nutrition. PIN4 expression levels were markedly decreased in roots of NC89 by sole and low NO3- nutrition, while they were unaffected in Zhongyan 100 roots. Overall, our findings suggest that LRs formation in tobacco seedlings is regulated by NH4+/NO3- ratios via modifying polar transport of auxin.

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