Position: Home > Articles > Study on the function of has B gene of Streptococcus equi ssp. zooepidemicus
Heilongjiang Animal Science and Veterinary Medicine
2016
(13)
163-166
马链球菌兽疫亚种hasB基因功能的研究
作 者：
付强;王爱富;罗惠娜;马春全
单 位：
佛山科学技术学院生命科学学院
关键词：
马链球菌兽疫亚种(Streptococcus equi ssp.zooepidemicus,SEZ);荚膜;has B基因;突变菌株;调理吞噬;致病机制
摘 要：
为了阐明马链球菌兽疫亚种(Streptococcus equi ssp.zooepidemicus,SEZ)荚膜的致病机理,使该菌引起的猪、马、牛等多种动物的链球菌病得到有效控制,试验采用Real-time PCR技术分析has B基因的体内表达特性,通过构建基因缺失突变菌株、调理吞噬试验等对has B基因的致病性进行系统评价。结果表明:相对于体外培养的细菌,SEZ感染小鼠脾脏分离细菌的mRNA表达均显著下调;基因缺失突变菌株Δhas B与亲本菌株的生长状况基本一致,但是致病力显著降低;基因缺失突变菌株Δhas B影响补体和巨噬细胞介导的免疫反应,使其抵抗宿主巨噬细胞的吞噬能力显著降低。
译 名：
Study on the function of has B gene of Streptococcus equi ssp. zooepidemicus
作 者：
FU Qiang;WANG Aifu;LUO Huina;MA Chunquan;School of Life Science,Foshan University;
关键词：
Streptococcus equi ssp;;zooepidemicus(SEZ);;capsule;;has B gene;;mutant strain;;opsonophagocytosis;;pathogenesis
摘 要：
To clarify the pathogenesis of Streptococcus equi ssp.zooepidemicus(SEZ) capsule,and effectively control streptococcal disease caused by it in pigs,horses,cattle,and other animals,Real-time PCR technique was used to analyze in vivo expression characteristics of has B gene.Systematic evaluation of the pathogenicity of has B gene was performed through constructing the gene-deletion mutant strain,opsono-cytophagic test,and so on.The results showed that,relative to bacteria cultured in vitro,mRNA expressions of isolated bacteria from spleen of mice infected by SEZ were significantly down-regulated.In addition,the growth of Δhas B as gene-deletion mutant strain was basically same as the parent strain,but its pathogenicity significantly reduced.Δhas B as gene-deletion mutant strain affected the complement and macrophage-mediated immunoreactions,causing a significant decrease in resisting the phagocytotic activity of host macrophages.
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