Extracted Method and Preliminary Structural Analysis of Red Sorghum Pigment

Li Zhuying1, Mao Shaochun1, Dai Rongzhen2, Sun Guilan1, Xie Xu1 (1Yuxi Agricultural vocational Institute, Yuxi Yunnan 653100; 2Yuxi City Hongta Seed Station, Yuxi Yunnan 653100)

sorghum pigment; extraction; structure; analyse

The best extracted method and structure of red sorghum pigment were studied to provide experimental date for the development and application. The effect factors of extracted rate of red sorghum pigment, such as chroma, acidity, temperature and extraction time was tested respectively by refluxing extracted method. The best extracted method of red sorghum pigment was selected by the orthogonal experiment (L934). Pigment solution of red sorghum and purple black sorghum was scanned at the wavelength range of 180-800 nm. The orthogonal experiment define that the best extraction method of pigment of red sorghum were 80℃,75% (v/v) alcohol, a 2.0 of pH value, the ratio of solid to liquid of 1:100 g/mL, extraction time of 2 h. The biggest absorption peak wavelength of pigment solution from red and purple black of sorghum is 494 nm and 499 nm respectively in the visible light area. Preliminary identify pigment of red sorghum includes the flavanones or flavanonols substance by analyzing the ultraviolet spectrograph data. Spectral date is different between red sorghum and purple black sorghum pigment solution in the visible light area, also different between red sorghum pigment and flavones or flavonols (rutin) solution.