Position: Home > Articles > Effect of Sea Cucumber Collagen Oligopeptide in Diabetic Wound Healing
Food and Nutrition in China
2017
(07)
71-75
海参胶原低聚肽对糖尿病小鼠术后伤口愈合的促进作用
作 者：
李林;李迪;徐腾;刘睿;任金威;陈启贺;李勇
单 位：
北京大学公共卫生学院
关键词：
海参胶原低聚肽;糖尿病;伤口愈合
摘 要：
目的:观察海参胶原低聚肽是否具有促进db/db 2型糖尿病小鼠术后伤口愈合的作用。方法:取90只10w龄的db/db小鼠分为5组,包括模型对照组乳清蛋白组和3个海参胶原肽组(从低到高分别为0.25、0.50、1.00g/kg·BW),每组18只。另设一组同周龄、性别的db/m小鼠18只作为正常对照组。模型对照组和正常对照组使用溶剂(蒸馏水)灌胃。进行背部切口手术后开始干预,分别于术后第4、7、14d分批处死动物,每组每次处死6只。术后观察不同时期伤口愈合情况,测定血清生化指标、皮肤切口抗张力强度以及进行皮肤组织HE染色。结果:与模型对照组相比,海参胶原肽剂量组小鼠血清IL-8水平降低,IL-10水平升高,NO水平升高,伤口抗张力强度提升,伤口愈合较好。结论:海参胶原低聚肽能够改善糖尿病小鼠术后营养状况,促进伤口愈合。
译 名：
Effect of Sea Cucumber Collagen Oligopeptide in Diabetic Wound Healing
作 者：
LI Lin;LI Di;XU Teng;LIU Rui;REN Jin-wei;CHEN Qi-he;LI Yong;School of Public Health,Peking University;
关键词：
sea cucumber oligopeptide;;diabetes mellitus;;wound healing
摘 要：
【Objective】To observe the effect of sea cucumber collagen oligopeptide on the wound healing of diabetic mice,and to provide experimental basis for the further study on the relationship between sea cucumber collagen oligopeptide and diabetic wound healing.【Method】Totally 90 db/db mice were divided into 5 groups,including model control group,whey protein group( 0. 50 g/kg·BW) and 3sea cucumber collagen oligopeptide dose groups( 0. 25,0. 50,1. 00 g/kg·BW). Each group included 18 mice. A group of 18 db/m mice with the same age and gender was selected as the blank control group. The model control group and the normal control group were treated with solvent. The intervention was carried out after the incision operation on the back of mice,and the animals were sacrificed at day 4,7 and 14,respectively. We observed the wound healing condition every day after operation. The serum biochemical indexes were measured. The tensile strength of the wound was measured and the skin HE staining was performed. 【Result】Compared with the model control group,the tension strength of sea cucumber oligopeptide dose groups increased significantly. Pathological report showed that neutrophil granulocyte in the sea cucumber peptide dose groups decreased while blood capillary and fibroblasts increased. After the intervention,the detection of serum inflammation index IL-8 significantly decreased( P < 0. 05) while IL-10 increased( P < 0. 05). NO level was significantly increased( P <0. 05). 【Conclusion】Sea cucumber collagen oligopeptide can enhance wound tensile strength,promote angiogenesis,reduce inflammation of diabetic mice,thereby promoting wound healing.
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