当前位置: 首页 > 文章 > 不同花色牡丹品种亲缘关系的RAPD-PCR分析 中国农业科学 2002,35 (5) 546-551
Position: Home > Articles > RAPD-PCR Analysis of Genetic Diversity of Different Colour 35 Tree Paeonia Cultivars Scientia Agricultura Sinica 2002,35 (5) 546-551

不同花色牡丹品种亲缘关系的RAPD-PCR分析

作  者:
陈向明;郑国生;孟丽
单  位:
合肥教育学院;山东农业大学生命科学学院
关键词:
牡丹品种;亲缘关系;RAPD分析
摘  要:
用RAPD PCR技术对 7个花色 35个牡丹品种进行基因组DNA多态性分析 ,34个随机引物扩增出 4 18个DNA片段 ,其中 337条谱带表现多态性 ,多态性检测率为 80 .6 % ,表明受试牡丹栽培品种之间富含遗传多态性 ,其中18个受试牡丹品种产生特异性遗传标记 ,这些特异标记对各个牡丹品种具有一定的鉴别价值。将扩增图谱利用UP GMA方法构建遗传聚类树状图 ,分析品种间遗传关系。相似系数 1.5将 35个牡丹品种划分为 2个遗传聚类组 ,相似系数 1.0将其划分为 4个遗传聚类组 ,不同相似系数的遗传聚类划分与花色之间并非完全未有相关性。来源相同花色一致的牡丹品种之间亲缘关系相对较近 ,但产地来源对牡丹品种的亲缘关系的影响比花色更为突出。
译  名:
RAPD-PCR Analysis of Genetic Diversity of Different Colour 35 Tree Paeonia Cultivars
作  者:
CHEN Xiang ming 1, ZHENG Guo sheng 2, MENG li 2 ( 1Hefei Education College,Hefei 230001; 2College of Life Science, Shandong Agicultural University,Taian 271018)
关键词:
Peony cultivars; Genetic polymorphism; RAPD analysis
摘  要:
Random amplified polymorphic DNA (RAPD) was used to analyze the genetic polymophism of 35 tree peony cultivars derived from seven different flower colour types. Thirty four primers amplified 418 DNA fragments which included special DNA fragments from 18 cultivars. It could also be used to differentiate all cultivars being tested and the UPGMA method may be used to analyze the genetic relationship. The results showed that 35 tree peony cultivars were divided into 2 cluster groups having a similarity of 1.5, and those remaining were divided into 4 cluster groups having a similarity of 1.0. The results confirmed that the flower colour has no relationship to the genetic groups, but the tree peony cultivers come from the same place where the relationship is similar. Therefore, The genetic background has little effect on the genetic relationship.

相似文章

计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊