中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 超声波辅助双水相法提取枸杞叶黄酮及其胰脂肪酶抑制活性 食品与机械 2024,40 (8) 144-151
Position: Home > Articles > Extraction of flavonoids from Goji(Lycium barbarum L.)leaves by ultrasonic-assisted two-phase aqueous method and the inhibitory activity of the extract on pancreatic lipase Food & Machinery 2024,40 (8) 144-151

超声波辅助双水相法提取枸杞叶黄酮及其胰脂肪酶抑制活性

作  者：
贾晓凤;高蝶;刘金旺;徐钰莹;范艳丽
单  位：
宁夏大学食品科学与工程学院
关键词：
枸杞叶;黄酮;超声波;双水相法;胰脂肪酶
摘  要：
[目的]开发一种安全、无毒且高效的枸杞叶黄酮类化合物提取方法.[方法]采用超声波辅助双水相法实现高得率、绿色的枸杞叶黄酮类化合物提取,并评价产物活性.通过单因素和Plackett-Burman(PB)试验考察各因素对黄酮提取效果的影响,选取聚乙二醇(PEG400)使用量、硫酸铵使用量、料液比3个因素通过响应面中心组合试验设计优化提取条件;通过胰脂肪酶活性抑制试验评价提取物活性.[结果]超声波辅助双水相法提取枸杞叶黄酮最佳工艺条件为:聚乙二醇PEG相对分子质量400,PEG质量分数28%、硫酸铵质量分数16%、超声功率250 W,超声处理时间40 min、超声处理温度40℃、料液比1∶30(g/g),该条件下所提取的枸杞叶黄酮提取物的总黄酮含量为(486.30±3.29)mg/g,其对胰脂肪酶活性具有显著抑制作用,半数抑制质量浓度(IC50)为1.06 mg/mL.[结论]最优提取条件下,超声波辅助双水相法所提取的枸杞叶黄酮得率高、溶剂残留低,该方法所得枸杞叶黄酮对胰脂肪酶具有良好的抑制作用.
译  名：
Extraction of flavonoids from Goji(Lycium barbarum L.)leaves by ultrasonic-assisted two-phase aqueous method and the inhibitory activity of the extract on pancreatic lipase
关键词：
Goji(Lycium barbarum L.)leaves%flavonoids%ultrasonic wave%two aqueous phase method%pancreatic lipase
摘  要：
[Objective]This study aimed to achieve high yield and green extraction of flavonoids from Goji(Lycium barbarum L.)leaves using ultrasonic assisted aqueous two-phase method,and to evaluate the activity of extract.[Methods]Single factor and PB(Plackett-Burman)experiments were conducted to investigate the influence of various factors on the extraction efficiency of flavonoids.Three parameters were chosen to optimize the extraction conditions using a Central Composite Design(CCD)of the response surface experiment:the amount of polyethylene glycol(PEG400),the amount of ammonium sulfate,and the solid-liquid ratio.The extract's activity was assessed using an activity inhibition test of pancreatic lipase.[Results]The optimal process conditions for ultrasonic-assisted aqueous two-phase extraction of flavonoids from Goji berry leaves were:28%polyethylene glycol 400,16%ammonium sulfate,ultrasonic power of 250 W,ultrasonic treatment time of 40 minutes,ultrasonic treatment temperature of 40℃,and solid-solid ratio of 1∶30(g/g).Under these conditions,the total flavonoid content of the extract was(486.30±3.29)mg/g,which had a significant inhibitory effect on pancreatic lipase activity,and the half inhibition concentration(IC50)was 1.06 mg/mL.[Conclusion]Under the optimal extraction conditions,the flavonoids extracted from L.barbarum leaves by ultrasonic-assisted aqueous phase method had high yield and low solvent residue,and the flavonoids from L.barbarum leaves obtained by this method had a good inhibitory effect on pancreatic lipase.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46