作 者：
郑静格;郭志芯;刘婷婷;毛连纲;张燕宁;张兰;蒋红云
单 位：
中国农业科学院植物保护研究所农业农村部农产品质量安全生物性危害因子(植物源)控制重点实验室
关键词：
反式肉桂醛;稻绿核菌;细胞超微结构;烟酰胺腺嘌呤二核苷酸-苹果酸脱氢酶;琥珀酸脱氢酶;三磷酸腺苷酶
摘 要：
为初步探究反式肉桂醛(TC)对稻绿核菌的抑菌机制,采用透射电子显微镜观察了TC处理后稻绿核菌菌丝细胞的超微结构,并测定了2、4、8、15及30μg/mL系列质量浓度TC对稻绿核菌细胞壁完整性的影响,以及其对烟酰胺腺嘌呤二核苷酸-苹果酸脱氢酶(NADMDHase)、琥珀酸脱氢酶(SDHase)和三磷酸腺苷酶(ATPase)活力的影响。结果显示:经4μg/mL的TC处理后,菌丝细胞内脂质体显著增多,线粒体结构变模糊。当TC质量浓度升高至30μg/mL时,可破坏菌丝细胞壁的完整性,且对NAD-MDHase活力的相对抑制率为44.3%,对SDHase活力的相对抑制率为76.7%;而Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase、Ca~(2+)-ATPase及Mg~(2+)-ATPase的活力随TC质量浓度升高呈U型变化,其中,4μg/mL的TC对ATPase活力的抑制效果最强,相对抑制率达78.4%以上。研究表明,细胞壁及线粒体可能是TC抑制稻绿核菌菌丝生长的作用靶点,具有进一步研究的价值。
译 名：
Effects of trans-cinnamaldehyde against Villosiclava virens on cell ultrastructure and activities of three respiratory enzymes
作 者：
ZHENG Jingge;GUO Zhixin;LIU Tingting;MAO Liangang;ZHANG Yanning;ZHANG Lan;JIANG Hongyun;Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture and Rural Affairs of the People's Republic of China;
关键词：
trans-cinnamaldehyde;;Villosiclava virens;;cell ulstrastructure;;nicotinamide adenine dinucleotide-malic dehydrogenase;;succinate dehydrogenase;;adenosine triphosphatase
摘 要：
The antifungal mechanism of trans-cinnamaldehyde(TC) against Villosiclava virens was investigated. The alteration of cell ultrastructure was observed using transmission electron microscope.The integrity of cell wall and activities of nicotinamide adenine dinucleotide-malic dehydrogenase(NAD-MDHase), succinate dehydrogenase(SDHase) and adenosine triphosphatase(ATPase) of V.virens were determined after treated with TC at the mass concentration of 2, 4, 8, 15 and 30 μg/mL. The results showed that the contents of lipidosome increased significantly and the structure of mitochondrion was indiscernible after treatment with TC at the mass concentration of 4 μg/mL. With the mass concentration of TC increasing to 30 μg/mL, the integrity of cell wall in V. virens was destroyed and the relative inhibition of NAD-MDHase and SDHase were reduced by 44.3% and 76.7%,respectively. Differently, the activities of Na~+-K~+-ATPase, Ca~(2+)-Mg~(2+)-ATPase, Ca~(2+)-ATPase and Mg~(2+)-ATPase fluctuated as U-curve with the concentration increase of TC. When the mass concentration of TC was 4 μg/mL, the highest inhibitive activity of TC on ATPase was the achieved among all the tested concentrations and the relative inhibition of TC on ATPase was higher than 78.4%. These results suggested that the cell wall and mitochondrion were the possible action targets for TC to inhibit the mycelial growth.
