单 位：
中国水产科学研究院黑龙江水产研究所;东北林业大学
关键词：
美国金鳟;同工酶;聚丙烯酰胺凝胶电泳;组织特异性;遗传多样性
摘 要：
本实验运用聚丙烯酰胺凝胶电泳法对养殖型美国金鳟眼(E)、心(H)、肾(K)、肝(L)、肌肉(M)五种组织的乙醇脱氢酶(ADH)、酯酶(EST)、葡萄糖-6-磷酸脱氢酶(G-6- PDH)、谷氨酸脱氢酶(GDH)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和过氧化物酶(POD)等7种同工酶进行电泳研究。结果显示：美国金鳟中ADH、EST、G-6-PDH、GDH、LDH、MDH、POD、SOD 7种同工酶都有不同程度的组织特异性；美国金鳟群体同工酶分析共记录18个基因座位,其中有8个基因座位为多态,等位基因总数为26个,从而得出多态座位百分数P为44．44％,位点有效等位基因数Ne=1．4444,表明美国金鳟群体遗传多样性偏高。
译 名：
Isozymes Analysis Of Oncorhynchus mykiss
作 者：
WU Yi~(1,2) YIN Hong-bin~1 SUN Zhong-wu~2 WANG Bing-qian~1 MA Xiao-long~2 (1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;2.College of Life Sciences,Northeast Forestry University,Harbin 150040,China)
关键词：
King Trout(Oncorh ynchus mykiss);;Isozymes;;Polyacrylamide gel electrophoresis;;Tissue specificity;;Genetic diversity
摘 要：
By using the vertical polyacrylamide gel electrophoresis,Oncorhynchus mykiss domestica- ted were studied.The patterns of expression in 7 multilous isozymicsystems consisting of ADH、EST、G-6-PDH、GDH、LDH、MDH|and POD were investigated in 5 different tissues(eye,heart,kidney, liver and muscle)of Oncorhvnchus mvkiss.The results showed that 7 isozymes of ADH、EST、G-6- PDH、GDH、LDH、MDH and POD in the Oncorhynchus mykiss have clearly tissue specificity.Among the 7 enzymes studied,21 genetic loci were detected.The percentage of polymorphie loci is 57.14% and the average value of allele is 1.4762.Both P and Ne were high in the fish.
