Position: Home > Articles > RAPD MARKER OF RESISTANCE GENE TO STRAIN GPV OF BYDV IN WHEAT ADDITION LINE Z1
Acta Phytopathologica Sinica
1999
(1)
抗大麦黄矮病毒GPV株系小麦异附加系Z1抗性基因的RAPD标记
作 者：
孙晓平;钱幼亭;周广和
单 位：
中国农业科学院植物保护研究所
关键词：
RAPD，大麦黄矮病毒，异附加系
摘 要：
经生物学方法鉴定，小麦－中间偃麦草异附加系Ｚ１高抗我国特有的大麦黄矮病毒ＧＰＶ株系，用１０４个随机引物对Ｚ１及其亲本中７９０２的基因组进行ＲＡＰＤ分析，发现其中的引物ＯＰＳ－１６能从Ｚ１中扩增１个约１．７Ｋｂ的特异性片段。用引物ＯＰＳ－１６扩增Ｚ１、中７９０２、中间偃麦草、中５，同样也从Ｚ１、中间偃麦草、中５中能稳定地扩增此１．７Ｋｂ片段。对Ｚ１的抗、感病单株的自交后代进行ＲＡＰＤ分析，发现在所测的抗病株的自交后代中能稳定地扩增此１．７Ｋｂ片段，而在感病株的自交后代中则不能扩增。
译 名：
RAPD MARKER OF RESISTANCE GENE TO STRAIN GPV OF BYDV IN WHEAT ADDITION LINE Z1
作 者：
Sun XiaopingQian YoutingZhou Guanghe (Institute of Plant Protection, CAAS, Beijing
100094)
关键词：
RAPD, barley yellow dwarf virus, the addition
line
摘 要：
The wheat-Elytrigia intermedia addition line Z1 was identified to be highly
resistant to strain GPV of BYDV, and this strain exists only in China. The genomes of resistant
lines and susceptible lines were analyzed by RAPD using 104 random primers. It was found
that primer OPS16 can amplify a specific 1.7 Kb fragment from genome of Z1.When Z1,
Zhong7902, Elytrigia intermedia and Zhong5 were amplified using primer OPS16, this specific
1.7 Kb fragment was also produced in Elytrigia intermedia, Zhong5 and Z1. When selfbred
progenies of resistant and susceptible Z1 individuals were amplified using primer OPS16, this
specific 1.7 Kb fragment was amplified in progenies of resistant individuals of Z1, but not in
progenies of susceptible individuals.
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