中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 拮抗菌AL7的鉴定及其生防特性的初步研究 植物保护 2018 (2) 53-60
Position: Home > Articles > Species identification and biocontrol characteristics of antagonistic bacterium AL7 Plant Protection 2018 (2) 53-60

拮抗菌AL7的鉴定及其生防特性的初步研究

作  者：
刘海洋;王琦;王伟;张仁福;韩万里;姚举
单  位：
新疆农业科学院植物保护研究所农业部西北荒漠绿洲作物有害生物综合治理重点实验室农业部库尔勒作物有害生物科学观测实验站;中国农业大学植物保护学院;新疆农业大学农学院
关键词：
拮抗菌;甲基营养型芽胞杆菌;抑菌活性物质;生物自显影分析
摘  要：
从新疆棉田中分离获得一株细菌AL7,利用形态观察、生理生化性状测定结合16SrDNA、gyrB序列分析对菌株AL7进行种属鉴定,测定了菌株AL7及其挥发性气体对棉花黄萎病菌Verticillium dahliae的拮抗能力,利用酸沉淀法粗提菌株AL7的抑菌活性物质,并测定了该活性粗提物对棉花黄萎病菌生长及其孢子萌发的拮抗能力,利用薄层层析法对活性物质进行了初步分离。结果表明,菌株AL7为甲基营养型芽胞杆菌Bacillus methylotrophicus,该菌株对棉花黄萎病菌具有极强的抑制作用,分泌在PDA平板中的抑菌物质对病原菌的抑制持效期达14d以上,该菌株产生的挥发性气体对棉花黄萎病菌的抑制效果达59.2%;菌株AL7的脂肽粗提物对棉花黄萎病菌抑制率达到82.8%,能够充分抑制大丽轮枝菌孢子的萌发,抑菌圈直径为36.1mm,生物自显影分析表明,菌株AL7分泌的抑菌活性物质Rf值约为0.75~0.83,该菌株在防治棉花黄萎病方面表现出较好的应用前景。
译  名：
Species identification and biocontrol characteristics of antagonistic bacterium AL7
作  者：
LIU Haiyang;WANG Qi;WANG Wei;ZHANG Renfu;HAN Wanli;YAO Ju;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis,Ministry of Agriculture,Scientific Observation and Experimental Station of Crop Pests in Korla,Ministry of Agriculture;College of Plant Protection,China Agricultural University;College of Agronomy,Xinjiang Agricultural University;
关键词：
antagonistic bacterium;;Bacillus methylotrophicus;;antifungal active ingredient;;bioautography analysis
摘  要：
The bacterial strain AL7 was isolated from the cotton field in Xinjiang.It was identified through morphologic observation,physiological and biochemical character determination and 16 S rDNA and gyrB gene sequence analysis.The antagonistic effect of strain AL7 and its volatile gas that suppresses Verticillium dahliae growth were tested by confrontation culture method.Anti-fungal active ingredients of strain AL7 were extracted by acidic precipitation.The antagonistic effect of the active ingredients suppressing V.dahliae growth and their spore germination activities were tested by Oxford-cup test and plating method.The active substances were preliminarily separated by thin-layer chromatography method.The results indicated that strain AL7,identified as Bacillus methylotrophicus,had strong antagonistic effect on the growth of V.dahliae,with an inhibition zone of 33 mm in diameter and a lasting period of 14 d.The antibacterial effect of its volatile gas was above 50%.The antibacterial effect of the active crude extracts of strain AL7 was above 80%,which could sufficiently suppress the spore germination of V.dahliae.The diameter of inhibition zone was 36.1 mm and the Rf value of the anti-fungal active ingredient was about 0.75 to 0.83.Our research suggests that strain AL7 has good development prospect for control of cotton Verticillium wilt.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46