中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 1个新水稻组成型启动子的克隆与功能鉴定 华中农业大学学报 2012,31 (2) 139-146
Position: Home > Articles > Isolation and characterization of a novel rice constitutive promoter Journal of Huazhong Agricultural University 2012,31 (2) 139-146

1个新水稻组成型启动子的克隆与功能鉴定

作  者：
魏晶;毛伟华;林拥军;陈浩
单  位：
华中农业大学作物遗传改良国家重点实验室/华中农业大学国家植物基因研究中心(武汉)
关键词：
水稻;组成型启动子;GUS报告基因;GUS染色;GUS定量
摘  要：
根据基因芯片数据库和RT-PCR验证得到1个高活性的水稻组成型表达基因(TIGR Locus:LOC-Os07g34589),用PCR技术从籼稻品种明恢63基因组中克隆得到其上游启动子PSUI1,长度为1 941bp;将其与β-glucuronidase(GUS)报告基因融合构建植物表达载体DX2181b-PSUI1,利用玉米Ubiquitin启动子融合GUS报告基因构建表达载体DX2181b-PUbi作为对照,通过根癌农杆菌(Agrobacterium tumefaciens)介导法将DX2181b-PSUI1和DX2181b-PUbi转化粳稻品种中花11。组织化学染色表明,含DX2181b-PSUI1的转基因植株中,GUS基因在幼苗期叶片、叶鞘、根,抽穗期叶片、叶鞘、茎秆、颖壳、雄蕊和成熟期的叶片、叶鞘、茎秆、胚、胚乳中均有表达,说明PSUI1为组成型启动子。对GUS表达活性进行定量分析表明,PSUI1启动子的活性约为玉米Ubiquitin启动子活性的1/3～1/2,但是PSUI1表现出了更好的表达稳定性。
译  名：
Isolation and characterization of a novel rice constitutive promoter
作  者：
WEI Jing MAO Wei-hua LIN Yong-jun CHEN Hao National Key Laboratory of Crop Genetic Improvement/National Center of Plant Gene Research(Wuhan), Huazhong Agricultural University,Wuhan 430070,China
关键词：
Oryza sativa;constitutive promoter;GUS reporter gene;GUS staining;quantitative analysis of GUS
摘  要：
A constitutive gene(TIGR Locus:Loc_Os07g34589) was obtained by combining our lab's database with RT-PCR verification.A novel rice constitutive promoter named PSUI1 with 1 941 bp was isolated using the genomic DNA of the indica rice variety Minghui63 based on a PCR method.To determine the expression strength and pattern of the novel constitutive promoter,the cloned promoter was fused with the β-glucuronidase(GUS) reporter gene to form plant expression vector DX2181b-PSUI1,and the maize ubiquitin promoter was also fused with GUS reporter gene to form expression vector DX2181b-PUbi as the control.Both DX2181b-PSUI1 and DX2181b-PUbi were transformed into japonica rice variety Zhonghua11 by Agrobaeterium-mediated method.GUS staining showed that GUS gene was expessed in different rice tissues and organs at different growth stages of DX2181b-PSUI1 transgenic rice plants.Quantitative analysis of GUS activity showed that the strength of maize ubiquitin promoter was 2-3 times higher than that of the PSUI1 promoter,but the expression of PSUI1 promoter exhibited much more stable than that of maize Ubiquitin promoter between transgenic rice lines.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46