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Position: Home > Articles > Study on the Shoot-tip Culture of Hydrangea macrophylla in vitro Northern Horticulture 2012 (9) 137-138

八仙花茎尖离体培养技术研究

作  者:
王忠武;建德锋
单  位:
吉林农业科技学院
关键词:
八仙花;离体培养;培养基
摘  要:
以八仙花带芽枝条为试材,采用茎尖离体培养技术对八仙花进行快繁研究。结果表明:把经过消毒灭菌的茎尖切割后转入芽诱导培养基MS+6-BA 1.0mg/L+NAA 0.1mg/L+GA31.0mg/L,萌发率达到85%;继代培养基采用MS+6-BA 0.5mg/L+NAA 0.04mg/L+腺嘌呤5.0mg/L,会具有较高的生长系数;生根培养基采用1/2MS+IBA 0.8mg/L+活性炭3g/L,能够完全生根成苗。
译  名:
Study on the Shoot-tip Culture of Hydrangea macrophylla in vitro
作  者:
WANG Zhong-wu,JIAN De-feng(Jilin Agricultural Science and Technology College,Jilin,Jilin 132101)
关键词:
Hydrangea macrophylla;culture in vitro;medium
摘  要:
Hydrangea macrophylla was chosen as test material,the rapid propagation technique of Hydrangea macrophylla by shoot-tip culture in vitro were researched.The results showed that the shoot-tip after disinfected were seeded into the bud induction medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+GA31.0 mg/L,and the germination rate reached 85%;Using MS+6-BA 0.5 mg/L+NAA 0.04 mg/L+adenine 5.0 mg/L as the continue medium could had higher growth factor;using 1/2MS+IBA 0.8 mg/L+ Activated carbon 3 g/L as rooting medium could fully take root into plants.

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