Position: Home > Articles > Genomic characterization of DNA-A and associated satellite DNA molecule of an isolate of Tomato yellow leaf curl China virus in Solanum aculeatissimum
Acta Phytopathologica Sinica
2007,37
(2)
138-143
刺茄中分离的中国番茄黄化曲叶病毒及伴随的卫星DNA分子的全基因组结构
作 者：
廖白璐;刘勇;谢艳;周雪平
单 位：
浙江大学生物技术研究所
关键词：
刺茄;中国番茄黄化曲叶病毒;菜豆金色花叶病毒属;卫星DNA;DNAβ
摘 要：
从云南砚山刺茄(Solanum aculeatissimum)上分离到病毒分离物Y322,全序列测定表明,Y322DNA-A全长2730个核苷酸,共编码6个ORF。基因组比较发现,Y322DNA-A与中国番茄黄化曲叶病毒(TYLCCNV)各分离物的同源性最高(88.3%～99.2%),而与其它双生病毒的同源性均在79.6%以下,表明Y322是TYLCCNV的一个分离物。利用DNAβ的特异性引物在Y322中扩增到DNAβ分子(Y322β),序列分析表明,其全长1331个核苷酸,与TYLCCNV伴随的DNAβ的同源性最高,达75.1%～93.1%,而与已报道的其它种类的DNAβ的同源性均低于55.4%。这是首次在刺茄中检测到中国番茄黄化曲叶病毒。
译 名：
Genomic characterization of DNA-A and associated satellite DNA molecule of an isolate of Tomato yellow leaf curl China virus in Solanum aculeatissimum
作 者：
LIAO Bai-lu1,LIU Yong1,2,XIE Yan1,ZHOU Xue-ping1(1Institute of Biotechnology,Zhejiang University,Hangzhou 310029,China;2Yunnan Tobacco Research Institute,Yuxi 653100,China)
关键词：
Solanum aculeatissimum;Tomato yellow leaf curl China virus;Begomovirus;satellite DNA;DNAβ
摘 要：
The virus isolate Y322 was obtained from Solanum aculeatissimum showing leaf curl and enation symptoms in Yanshan,Yunnan Province.Complete nucleotide sequence of the DNA-A like molecule of Y322 was determined to be 2 730 nucleotides.Y322 DNA-A was most closely related to isolates of Tomato yellow leaf curl China virus(TYLCCNV)with 88.3%-99.2% nucleotide sequence identities,while less than 79.6% nucleotide sequence identities were found in comparisons with other begomoviruses.Therefore,Y322 was taxonomically considered as an isolate of TYLCCNV.Further studies showed that Y322 was associated with a DNAβ molecule.The DNAβ of Y322 contained 1 331 nucleotides having the highest nucleotide sequence identity(75.1%-93.1%)with DNAβ associated with TYLCCNV,while less than 55.4% nucleotide sequence identities were found in comparisons with other DNAβ molecules.This is the first report of TYLCCNV in S.aculeatissimum.
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