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Position: Home > Articles > Study of Chitin Extraction and Protein Recovery from Penaeus Vannawei Shrimp Shell Hubei Agricultural Sciences 2015,54 (15) 3731-3734

南美白对虾干虾壳甲壳素提取工艺比较及蛋白质回收

作  者:
孙茜;夏琪;叶颖;陈雨兰;唐红枫
单  位:
湖北省疾病预防控制中心;武汉东湖学院生命科学与化学学院
关键词:
南美白对虾(Penaeus vannamei);干虾壳;甲壳素;蛋白质;回收
摘  要:
以南美白对虾(Penaeus vannamei)干虾壳为原料,采用5种方法提取甲壳素,同时对蛋白质进行回收,得出从干虾壳中提取制备食品级甲壳素最优方案。结果表明,方法Ⅴ为最优方案,即虾壳预处理为5 mm大小,10%柠檬酸浸泡13 h,10%Na OH于97℃处理3 h,再经过10%H2O280℃水浴浸泡2 h氧化脱色,处理过程中料液比(m∶V,g∶m L)均为1∶10,可制得残留氮量2.20%、灰分含量1.33%、产率为25.16%的食品级甲壳素。产生的废液采用乙醇沉淀法回收蛋白质可减少污染,节约成本。
译  名:
Study of Chitin Extraction and Protein Recovery from Penaeus Vannawei Shrimp Shell
作  者:
SUN Qian;XIA Qi;YE Ying;CHEN Yu-lan;TANG Hong-feng;Hubei Provincial Center for Disease Control and Prevention;College of Life Science and Chemistry,Wuhan Donghu University;
关键词:
Penaeus vannamei;;dried shrimp shell;;chitin;;protein;;recycling
摘  要:
Taking Penaeus vannamei as raw material, five chitin extracting methods,Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,were researched to determine the optimal scheme of food-grade chitin extraction and preparation. The results showed that the method Ⅴ was the best extraction process. Namely that 5 mm of dried shrimp shell should be soaked for 13 h in 10% citric acid,treated for 3 h with 10% Na OH at 97 ℃, and then take a 80 ℃ water bath in 10% H2O2 for 2 h for oxidative decoloration,with solid-liquid ratio always 1 ∶10.Under this condition,the yield of food-grade chitin, containing residual nitrogen 2.20% and ash content1.33%,was 25.16%. Moreover,proteins were recycled from the waste liquid by ethanol precipitation techniques to reduce pollution and save cost.

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