单 位：
北京农学院植物科学技术学院;北京农学院园林学院/北京乡村景观规划设计工程技术研究中心/城乡生态环境北京实验室
关键词：
‘西伯利亚’百合;花香;单萜;Li-mTPS表达
摘 要：
使用动态顶空法采集‘西伯利亚’百合不同时间点(7:30、9:30、11:30、13:30、15:30、17:30、19:30)释放的香气,采用自动热脱附-气相色谱/质谱联用(ATD-GC/MS)技术分析鉴定花香成分及释放量。结果表明:单萜释放量呈现先增后减的规律,11:30、13:30、15:30时的释放量较高。在所有的花香成分中,罗勒烯、芳樟醇和β-月桂烯的释放量最高,它们是‘西伯利亚’百合主要的单萜花香化合物。进一步研究发现,‘西伯利亚’百合花瓣中的Li-mTPS(一种百合单萜合成酶基因)的表达与单萜释放表现相似的规律。可见,Li-mTPS的表达在‘西伯利亚’百合主要的单萜释放中起着关键作用,而调控机制有待于进一步研究。
译 名：
Diurnal Changes of Monoterpenes Emission and Li-mTPS Expression in Lilium ‘siberia'
作 者：
LI Tianjiao;HU Zenghui;ZHENG Jian;LENG Pingsheng;YANG Kai;College of Landscape Architecture,Beijing University of Agriculture,Beijing Engineering Research Center of Rural Landscape Planning and Design,Beijing Laboratory of Urban and Rural Ecological Environment;College of Plant Science and Technology,Beijing University of Agriculture;
关键词：
Lilium ‘siberia';;Floral scent;;Monoterpene;;Li-mTPSexpression
摘 要：
Monoterpenes are an important category of volatile compounds in the floral scent.In this study,using dynamic headspace method,the floral scent emitted fromLilium ‘siberia'flowers was collected at different time points(7:30,9:30,11:30,13:30,15:30,17:30,19:30)during the daytime,then the components and release amounts were identified and analyzed by the automated thermal desorption-gas chromatography/mass spectrometry(ATD-GC/MS)technique.We found that release amounts of monoterpenes first increased and then decreased during the daytime,and a high level occurred at 11:30,13:30,and 15:30.The high release amounts of ocimene,linalool,andβ-myrcene indicated that they were the dominant monoterpenes in the floral scent of Lilium ‘siberia'.In the following study,the daytime expression of Li-mTPS(aLilium monoterpene synthase gene)in tepals was examined.Li mTPSexpression showed a similar pattern with monoterpene emission.The results showed that the Li-mTPS expression may play a key role in monoterpene emission from Lilium‘siberia'flowers,but the mechanism needs to be investigated in the future study.
