当前位置: 首页 > 文章 > 大蓟提取物对植物病原真菌的抑菌活性成分研究 农药 2013,52 (7) 529-532
Position: Home > Articles > Anti-Phytopathogenic Fungi Active Constituent in Extract from Cirsium japonicum DC. Agrochemicals 2013,52 (7) 529-532

大蓟提取物对植物病原真菌的抑菌活性成分研究

作  者:
魏朝霞;姚茹瑜;唐嘉义
单  位:
云南农业大学生物多样性研究与应用技术国家工程中心
关键词:
大蓟;植物提取物;抑菌活性;半数有效浓度;活性成分
摘  要:
[目的]大蓟有广泛的生物活性,具有生物农药的开发潜力。确定其中抗植物病原菌的主要活性成分,以期为其在农药领域的开发利用提供依据。[方法]通过溶剂提取法提取,用生长速率法测定提取物对5种植物病原真菌的抑菌活性,并用柱层析法分离活性物质。[结果]在质量浓度50 g/L时石油醚提取物对石榴枯萎病菌的抑菌活性最高,抑制率达100%,EC50值为5.1 g/L;从中分离出一个三萜类单体化合物,经鉴定为3β,21β-dihydroxyl-20(30)-en-taraxastane。[结论]大蓟对植物病原真菌有良好的抑菌活性,所分离到的单体化合物为大蓟中的主要抑菌活性成分。
译  名:
Anti-Phytopathogenic Fungi Active Constituent in Extract from Cirsium japonicum DC.
作  者:
WEI Zhao-xia,YAO Ru-yu,TANG Jia-yi(The National Center Agro-biodiversity,Yunnan Agricultural University,Kunming 650201,China)
关键词:
Cirsium japonicum DC.;plant extracts;antimicrobial activity;EC50;active components
摘  要:
[Aims] Cirsium japonicum DC.has a wide-spectrum biological activity and possess exploitation potential to be biopesticide.The research aims to explore the main antifungal component to provide basis for the developent and utilization in pesticide field.[Methods] Solvent extraction method was used to extract active components.A growth rate method was used for determining the antifungal activity of the extraction against 5 kinds of pathogens,and active material was separated by Column Chromatography.[Results] At 50 g/L,petroleum ether extract has the best inhibitory effect on Ceratocystis fimbriata with inhibition rate of 100% and with EC50 value of 5.1 g/L.A triterpene monomer compound was separated and identified as 3β,21β-dihydroxyl-20(30)-en-taraxastane.[Conclusions] Daji has an excellent antifungal activity on plant pathogenic fungi,and the monomer compound is the main antifungal component in Daji.

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