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Position: Home > Articles > Identification of tobacco bacterial wilt pathogen in Shaanxi Acta Tabacaria Sinica 2014 (5) 87-92

陕西烟草青枯病的发生与病原鉴定

作  者:
成巨龙;罗晶;邹航;柯美福;牛俞德;安德荣
单  位:
陕西省烟草公司;西北农林科技大学
关键词:
烟草青枯病;病原鉴定;青枯劳尔氏菌
摘  要:
2013年7月在陕西省西乡县韩岭村烟田首次发现疑似烟草青枯病病株,为了解陕西烟草青枯病的发生危害,对陕西省不同烟区的烟草青枯病的发生情况进行系统调查,并对田间采集的具有典型青枯病症状的烟草茎部样本进行了病原鉴定。结果表明:陕西省烟草青枯病发生区域为陕南的汉中、安康和商洛3个烟区,延安、宝鸡和咸阳的三个烟区尚未发现。发病始于6月上旬,盛发于7月中旬。该病害随温度升高而病情加剧,发病早期田间死苗严重,发病率可高达24.67%,而且有蔓延扩展趋势;通过对烟草茎秆上分离的病原细菌进行形态观察、致病性测定、生理生化性状等常规细菌鉴定比较,结合细菌16S rDNA序列分析,鉴定该病原菌为青枯劳尔氏菌Ralatonia solanacearum。
译  名:
Identification of tobacco bacterial wilt pathogen in Shaanxi
作  者:
CHENG Julong;LUO Jing;ZOU Hang;KE Meifu;NIU Yude;AN Derong;Shaanxi Provincial Tobacco Company;State Key Laboratory of Crop Biology Adversity in Arid Regions, Northwest Agriculture and Forestry University;
关键词:
tobacco bacterial wilt;;identification of pathogen;;Ralatonia solanacearum
摘  要:
Tobacco bacterial wilt in Shaanxi was investigated using samples typical of wilt symptoms. Results showed that bacterial wilt occurred in tobacco growing areas of southern Shaanxi province including Hanzhong, Ankang and Shangluo. No bacterial wilt disease was observed in other tobacco growing areas such as Yan'an, Baoji and Xianyang. It broke out in early June and prevailed in the middle of July, indicating that it aggravated in line with rising temperature( with incidence of up to 24.67%). Morphology observation, pathogenicity determination, physiological and biochemical identification in combination with 16 S rDNA sequence analysis showed that the pathogen was Ralatonia solanacearum.

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