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当前位置: 首页 > 文章 > 暹罗斗鱼致病性嗜水气单胞菌的分离·鉴定及药敏试验 安徽农业科学 2012,40 (22) 155-158
Position: Home > Articles > Isolation,Identification and Drug Sensitivity Test of Pathogenic Aeromonas hydrophila from Betta splendens Regan Journal of Anhui Agricultural Sciences 2012,40 (22) 155-158

暹罗斗鱼致病性嗜水气单胞菌的分离·鉴定及药敏试验

作  者：
杨宁;黄海;张希;魏赟;巫火连
单  位：
海南省三亚市节能监察中心;海南省三亚市南繁科学技术研究院
关键词：
暹罗斗鱼;嗜水气单胞菌;半致死量(LD50);药敏试验
摘  要：
[目的]探讨暹罗斗鱼的发病原因和病害防治方法。[方法]对暹罗斗鱼的病原菌进行分离,并通过生理生化指标测定和16S rD-NA测序对其鉴定,同时进行药敏试验。[结果]菌株DY001为暹罗斗鱼嗜水气单胞菌病的病原菌。经鉴定,确定菌株DY001为嗜水气单胞菌(Aeromonas hydrophila)。该病原菌对暹罗斗鱼的半致死量(LD50)为0.04×106CFU/g。药敏试验结果表明,病原菌对阿奇霉素、奥复星、氟哌酸、复方新诺明、菌必治、甲氧苄啶和氯霉素等抗生素敏感。[结论]嗜水气单胞菌是引起此次暹罗斗鱼发病的病原菌,而药敏试验结果将为其防治提供参考。
译  名：
Isolation,Identification and Drug Sensitivity Test of Pathogenic Aeromonas hydrophila from Betta splendens Regan
作  者：
YANG Ning et al(Sanya Science-Technology Academy for Crop Winter Multiplication,Sanya,Hainan 572000)
关键词：
Betta splenders Regan;Aeromonas hydrophila;LD50;Drug sensitivity test
摘  要：
[Objective] The research aimed to discuss the disease cause of Betta splenders Regan and the prevention and control methods of its diseases and pests.[Method] The pathogenic bacteria of B.splenders were isolated and identified by determining the physiological and biochemical indices and 16 S rDNA sequencing.And the drug sensitivity test was made.[Result] Strain DY001 was the pathogenic bacteria that caused Aeromonas hydrophila disease of B.splenders.Strain DY001 was identified as Aeromonas hydrophila.The median lethal dose(LD50) of this pathogeny to B.splenders was 0.04×106 CFU/g.The results of drug sensitivity test showed that this pathogenic bacteria was sensitive to azithromycin,ofloxacin,norfloxacin,compound sinomin,ceftriaxone,trimethoprim,chloramphenicol and other antibiotics.[Conclusion] Aeromonas hydrophila was the pathogenic bacteria that caused the disease incidence of B.splenders.The results of drug sensitivity test could provide references for the disease prevention and control of B.splenders.

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