关键词：
复合菌系MC1;谷子秸秆;生物量;分解产物
摘 要：
[目的]本文旨在探索高效纤维素分解复合菌系MC1及碱预处理对谷子秸秆消化过程的影响.[方法]通过监测15d发酵过程中消化液的性质变化,评价MC1对谷子秸秆酸化前处理的效果.[结果]结果表明,复合菌系MC1能有效分解谷子秸秆,碱处理及未处理秸秆15d的总减重分别达到61.8%和60.0%.与未处理秸秆相比,碱处理秸秆0~3d的总减重高出23.33%.秸秆分解过程中胞外木聚糖酶活高于羧甲基纤维素酶活,未处理秸秆为底物时的生物量高于碱处理秸秆.秸秆分解第3天培养液中的乙酸浓度达到最高,碱处理及未处理秸秆为底物时浓度分别为1.27g·L-1、1.18g·L-1.[结论]综上,MC1能够高效分解谷子秸秆,对秸秆资源的转化具有开发利用潜力.
作 者：
Cao Yanzhuan;Zhang Jianwei;Yan Shuangdui;College of Resources and Environment,Shanxi Agricultural University;
单 位：
Cao Yanzhuan%Zhang Jianwei%Yan Shuangdui%College of Resources and Environment,Shanxi Agricultural University
关键词：
Composite microbial system MC1;;Millet straw;;Biomass;;Decomposition products
摘 要：
[Objective]The study was to explore the effects of both MC1 and alkaline pretreatment on the degradation process of millet straw.[Methods]Millet straw were soaked into one percent alkaline(NaOH)for 24 h,and the effect of the alkaline pretreatment was evaluated through chemical composition analysis of the pretreated and non-treated biomass samples.The effect of MC1 on degradation rate of alkaline treated and non-treated millet straw was determined by evaluating the properties of degraded products in the fermentation broth.[Results]The results showed that the degradation rates of alkali treated and non-treated millet straw were 61.8%and 60.0%,respectively,after 15 days cultivation at 50 ℃.Compared to non-treated straw,the total biomass loss of alkali pretreated straw was enhanced by23.33%in 3 days cultivation.The activity of extracellular xylanase was constantly higher than that of CMCase during the full process.The concentration of acetic acid in the fermentation broth was reached at the highest level in the third day of the decomposition with 1.27 g/l for the alkali treatment straw and 1.18 g/l for the non-treated millet straw.[Conclusion]In conclusion,the composite microbial system MC1 showed the potential to be used for millet straw degradation.
