中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 冷冻速率和解冻温度对猪精液冷冻效果的影响 中国畜牧兽医 2012,39 (11) 157-160
Position: Home > Articles > Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen China Animal Husbandry & Veterinary Medicine 2012,39 (11) 157-160

冷冻速率和解冻温度对猪精液冷冻效果的影响

作  者：
梁鸿斌;姚学军;李维华;邵慧;朱金清;邬纯鸿;倪和民;刘云海;郭勇
单  位：
北京市昌平区种猪场;北京农学院动物科学技术学院;北京奶牛中心;北京市昌平区动物疫病预防控制中心
关键词：
猪;精液冷冻;细管;冷冻速率;体外受精
摘  要：
为优化冷冻和解冻方法,提高冷冻效果,本试验比较了不同冷冻速率(-100℃10min、-120℃10min、-140℃10min)和不同解冻温度(37℃30s、45℃30s、52℃30s、60℃30s)对猪精液冷冻效果的影响。结果表明,采用-120℃熏蒸10min,解冻后精子活力为0.36,质膜完整率和顶体完整率也优于其他2组,且差异显著(P<0.05)。采用37℃30s方法解冻,精子活力、质膜完整率显著高于其他3组,顶体完整率也高于其他3组,但差异不显著(P>0.05),其畸形率最低和60℃30s组差异明显(P<0.05),但与45℃30s组和52℃30s组差异不显著(P>0.05)。因此,采用-120℃平衡10min冷冻,37℃30s水浴解冻方法更为适合0.25mL细管猪冻精解冻。
译  名：
Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen
作  者：
LIANG Hong-bin1,YAO Xue-jun4,LI Wei-hua1,SHAO Hui1,ZHU Jin-qing3,WU Chun-hong1,NI He-min2,LIU Yun-hai2,GUO Yong2(1.Beijing Dairy Cattle Center,Beijing 100192,China;2.College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China;3.Beijing City of Changping Pig Breeding Farm,Beijing 102212,China;4.Beijing City Changping District Animal Disease Prevention and Control Center,Beijing 102200,China)
关键词：
boar;cryopreservation;dtraw;freezing frequency;artificial insemination
摘  要：
For improving the freezing and de-freezing methods for boar semen further,it was aimed to investigate the effect of different freezing frequency(-100 ℃ 10 min,-120 ℃ 10 min,-140 ℃ 10 min) and thawing temperature(37 ℃ 30 s,45 ℃ 30 s,52 ℃ 30 s,60 ℃ 30 s) on cryopreservation of boar semen.The results showed that the sperm motility,plasma integrity,normal acrosome rate by freezing treatment with-120 ℃ 10 min,were all significantly higher than those of the other three groups respectively(P<0.05).The sperm motility,plasma integrity,normal acrosome rate by thawing treatment with 37 ℃ 30 s,were not significantly higher than those of the other three groups respectively(P>0.05),but the difference of the sperm abnormality in the treatment with 60 ℃ 30 s was significant(P<0.05),but with 45 ℃ 30 s and 52 ℃ 30 s were not significant(P>0.05).Therefore,it could be concluded that the combination of freezing by-120 ℃ 10 min and thawing by 37 ℃ 30 s were appropriate to frozen-thawed 0.25 mL pipettes of boar semen.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46