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Position: Home > Articles > Preparation, Purification and Characterizations of Monoclonal Antibody Against Okadaic Acid Chinese Journal of Veterinary Science 2006,26 (6) 639-641

抗大田软海绵酸单克隆抗体的制备、纯化及其特性

作  者:
于光;卢士英;李岩松;周玉;任洪林;谭建华;柳增善
单  位:
吉林大学教育部人兽共患病重点实验室;吉林进出口检验检疫局;军事医学科学院军事兽医研究所
关键词:
大田软海绵酸;单克隆抗体;纯化;亲和常数
摘  要:
用大田软海绵酸与人IgG的偶联物OA-IgG作为免疫原,免疫BALB/c小鼠,通过常规细胞融合技术将免疫脾细胞与小鼠骨髓瘤细胞系SP2/0融合,用间接竞争ELISA方法对融合细胞所分泌抗体的特异性进行筛选。经过3次克隆,获得1株可稳定分泌抗OA的杂交瘤细胞株3C5,其染色体数为50±2,所分泌抗体属IgG1亚类,相对分子质量是159390,抗体亲和常数为9.8×108L/mol,滴度达2.56×106。
译  名:
Preparation, Purification and Characterizations of Monoclonal Antibody Against Okadaic Acid
作  者:
YU Guang1, LU Shi-ying2, LI Yan-song2, ZHOU Yu2, REN Hong-lin2, TAN Jian-hua3,LIU Zeng-shan 2* (1.The Imports and Exports Quarantine Bureau of Jilin Province,Changchun 130062, China; 2. Zoonosis Institute, Jilin University, Changchun 130062,China; 3. The Military Veterinary Institute,Academy of Military Medical Sciences, Changchun 130062,China)
关键词:
okadaic acid (OA); monoclonal antibody(McAb); purification; affinity constant
摘  要:
Spleen cells from mice hyperimmunized with a human IgG-okadaic acid N,N-dicyclohexyl carbodiimide conjugate were fused with SP2/0 myeloma cells. One hybridoma cell line was identified that secretes IgG1 monoclonal antibody, designated 3C5, against okdacid acid, after being subcloned for 3 cycles by indirect ELISA. It′s average number of the chromosome was 50±2. The molecular weight, the affinity constant and the titer of the McAb was 159 390, 9.8×108 L/mol and 2.56×106 respectively.

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