Position: Home > Articles > Morphological and molecular characterization of Xiphinema brevicollumfrom Shanxi
Plant Protection
2015
(5)
134-139
山西短颈剑线虫(Xiphinema brevicollum)形态与rDNA分子特征
作 者：
霍世英;徐玉梅;高俊明;王建明;赵增旗
单 位：
新西兰皇家研究院土地环境研究所;山西农业大学农学院
关键词：
剑线虫;形态;ITS1;ITS2;28SRNA
摘 要：
采用浅盘分离法在山西太谷松树根际土壤中分离得到一种剑线虫,通过Seinhorst脱水制片后利用显微镜观察其形态特征并进行测量,其主要特征为:雌性虫体经热杀死后呈腹面弯曲的"C"形,体长1.71~2.26mm,两端渐细;齿针全长127.3~140.8μm;a(体长/体宽)为35.9~51.4μm,b(体长/食道外宽)为5.8~8.7μm,c(体长/尾长)为50.7~103μm。前卵巢与后卵巢长短相近,尾形近似圆锥形。未见雄虫。对该线虫的rDNA-ITS1,rDNAITS2和28SRNA序列进行扩增、测序及系统进化分析,结合形态学特征进行鉴定,将分离到的剑线虫鉴定为短颈剑线虫(Xiphinema brevicollum),这是山西首次发现报道剑线虫属的一个种。
译 名：
Morphological and molecular characterization of Xiphinema brevicollumfrom Shanxi
作 者：
Huo Shiying;Xu Yumei;Gao Junming;Wang Jianming;Zhao Zengqi;College of Agriculture,Shanxi Agricultural University;Landcare Research,Private Bag 92170,Auckland Mail Centre;
关键词：
Xiphinema;;morphology;;ITS1;;ITS2;;28S RNA
摘 要：
In the present study,soil samples were collected from rhizosphere soil of pine trees in Taigu County of Shanxi Province.Nematodes were extracted from soil samples by a modified Baermann funnel method.Specimens used for morphological and morphometric studies were prepared as permanent slides using the Seinhorst's method.Microscopic observation demonstrated that the female body was usually strongly curved,C-shaped,with the body length of 1.71-2.26 mm and the entire odontostyle length of 127.3-140.8μm.a(body length/body diameter at mid-body),b(body length/pharyngeal bulb diam.)and c(body length/tail length)were 35.9-51.4μm,5.8-8.7μm,50.7-103μm,respectively.The length of anterior uterus was similar to the posterior one.Tail regularly was presented to be short conical.Male was not observed.Based on the morphological characteristic and sequences of rDNA-ITS1,rDNA-ITS2 and 28 S RNA,the nematode was identified to be Xiphinema brevicollum(Nematoda:Longidoridae),which was firstly reported in Shanxi Province.
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