当前位置: 首页 > 文章 > 金属离子对霍山石斛类原球茎增殖及植株再生的影响 园艺学报 2006,33 (1) 179-181
Position: Home > Articles > Effects of Metal Ions on Protocorm-like Body Proliferation in Liquid Culture of Dendrobium huoshanense and Plantlet Regeneration Acta Horticulturae Sinica 2006,33 (1) 179-181

金属离子对霍山石斛类原球茎增殖及植株再生的影响

作  者:
查学强;罗建平;石玮;姜绍通
单  位:
合肥工业大学生物与食品工程学院
关键词:
霍山石斛;类原球茎;金属离子;液体培养;植株再生
摘  要:
采用单因素浓度试验和正交试验研究了Mg2+、Ca2+、Fe2+、Mn2+和Zn2+离子对液体培养霍山石斛类原球茎增殖的影响。结果表明,5种金属离子对类原球茎增殖影响的主次顺序为Fe2+>Mn2+>Mg2+>Ca2+>Zn2+,促进类原球茎增殖的优化组合为Mg2+1.5 mmol.L-1、Ca2+4.5 mmol.L-1、Fe2+0.1mmol.L-1、Mn2+0.5 mmol.L-1、Zn2+0.06 mmol.L-1。经优化的液体培养基增殖的类原球茎能保持植株再生能力,平均每克类原球茎增殖后可再生植株1 985.2株,是相同时间内对照类原球茎再生植株数的4.2倍。
译  名:
Effects of Metal Ions on Protocorm-like Body Proliferation in Liquid Culture of Dendrobium huoshanense and Plantlet Regeneration
作  者:
Zha Xueqiang,Luo Jianping~*,Shi Wei,and Jiang Shaotong(Department of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China)
关键词:
Dendrobium huoshanense;Protocorm-like bodies(PLBs);Metal ion;Liquid culture;Plantlet regeneration
摘  要:
This research is concerned with optimization of Mg~(2+),Ca~(2+),Fe~(2+),Mn~(2+) and Zn~(2+) for protocorm-like body proliferation in liquid culture of Dendrobium huoshanense by one factor-at-a-time and orthogonal matrix methods.Results showed that,except for Zn~(2+),the effects of other metal ions on PLB proliferation were significant and the order was Fe~(2+)>Mn~(2+)>Mg~(2+)>Ca~(2+)>Zn~(2+).The optimal concentration combination for PLB proliferation was determined as 1.5 mmol·L~(-1) Mg~(2+),4.5 mmol·L~(-1) Ca~(2+),0.1 mmol·L~(-1) Fe~(2+),0.5 mmol·L~(-1) Mn~(2+) and 0.06 mmol·L~(-1) Zn~(2+).Proliferated PLBs remained the potential of plant regeneration.The amount of regenerated plantlets from per gram PLBs through proliferation in optimized liquid medium was 1 985.2 and was about 4.2 times that from PLBs that proliferated on original medium.

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