Position: Home > Articles > Study on the Constituents of Sn-2 Fatty Acid from Milk Triacylglyceride
Journal of Dairy Science and Technology
2009,32
(2)
24-26
牛乳脂肪甘油三酯中sn-2位脂肪酸组成的分析
作 者：
韩瑞丽;马健;张佳程;生庆海
单 位：
东北农业大学黑龙江省乳品工业技术开发中心;河北省乳业工程技术研究中心;青岛农业大学食品学院
关键词：
牛乳脂肪;甘油三酯;sn-2位脂肪酸;高效液相-蒸发光散射检测器;气质联用
摘 要：
用胰脂酶专一水解掉牛乳脂肪甘油三酯sn-1和sn-3位置上的脂肪酸得到sn-2单甘油酯和游离脂肪酸,再通过高效液相-蒸发光散射检测法分离出sn-2位单甘油酯,甲酯化后,用GC-MS对sn-2脂肪酸组成进行分析。结果表明石家庄地区夏季牛乳脂肪sn-2位脂肪酸主要有六种,分别为:月桂酸2.57%、豆蔻酸7.68%、棕榈酸34.74%、亚油酸11.56%、油酸22.53%、硬酯酸15.21%。
译 名：
Study on the Constituents of Sn-2 Fatty Acid from Milk Triacylglyceride
作 者：
Han Ruili1,Ma Jian2,Zhang Jiacheng1,Sheng Qinghai3 (1 The Food Science and Engineering of Qingdao Agriculture University,Qingdao of Shandong 266109,P. R. China;2 Hebei Province Dairy Engineering and Technology Research Center; 3 Heilongjiang Dairy Industry Technical Development Center of Northeast Agricultural University)
关键词：
milk fat; TAG; sn-2 fatty acid; HPLC-ELSD; GC-MS
摘 要：
2-Monoacyl-sn-glycerols and free fatty acids were obtained from milk TAG upon hydrolysis of the acyl group in the 1-and 3-position of glycerol using porcine pancreatic lipase. Then 2-Monoa-cyl-sn-glycerols was separated by HPLC-ELSD,the 2-position fatty acid composition was determined by GC-MS of the fatty acid methyl esters. The results shows that 2-position fatty acid of milk were composed by Lauric acid 2.57%,Myristic acid 7.68%,Palmitic acid 34.74%,Linolenic acid 11.56%,Oleic acid22.53% and Stearic acid 15.21%.
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