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Position: Home > Articles > Preparation and properties of chondroitin sulfate from Lophius litulon bones Science and Technology of Food Industry 2010 (12) 258-260

鮟鱇鱼硫酸软骨素的提取及性质研究

作  者:
张小军;张虹
单  位:
浙江省海洋水产研究所;浙江工商大学
关键词:
鮟鱇鱼;硫酸软骨素;提取;分析
摘  要:
建立了从鮟鱇鱼骨中提取硫酸软骨素的方法,并对提取工艺进行了优化。样品用20%氯化钠碱溶液浸提,pH7·8条件除去中性蛋白,pH2·5除去酸性蛋白,然后用70%(v/v)乙醇沉淀获得鮟鱇鱼硫酸软骨素。本方法硫酸软骨素的得率为3·47%,所得鮟鱇鱼硫酸软骨素产品为白色粉末,纯度为90·94%。对鮟鱇鱼硫酸软骨素的理化性质研究表明,其水分含量为8·88%,氮含量为2·86%,氨基己糖含量为30·12%,葡萄糖醛酸含量为31·40%。从鮟鱇鱼骨中提取出的硫酸软骨素产品电泳实验显示为单点,表明其为单一糖胺聚糖,红外光谱吸收特征显示其为硫酸软骨素C。
译  名:
Preparation and properties of chondroitin sulfate from Lophius litulon bones
作  者:
ZHANG Xiao-jun1,2,ZHANG Hong1,*(1.Zhejiang Gongshang University,Hangzhou 310035,China;2.Zhejiang Marine Fisheries Research Institute,Zhoushan 316100,China)
关键词:
Lophius litulon;chondroitin sulfate;extraction;analysis
摘  要:
A method of preparation of chondroitin sulfate from Lophius litulon bones was studied.Briefly,sample were extracted with 20% NaCl,and the solution was removed neutral protein at 90℃,pH7.8,then centrifuging to remove the acidic protein again at pH2.5,finally using 70%(v/v)enthanol to deposit the CS.The yield of this method was 3.47%,and the chondroitin sulfate product was white power,the purity was 90.94%.The CS physicochemical analysis showed that the contents of moisture,nitrogen,hexosamine and uronic acid were 8.88%,2.86%,30.12%,31.40%,respectively.The results of agarose gel electrophoresis reflected that the chondroitin sulfate from Lophius litulon was purified.The IR spectrum showed that chondroitin sulfate from Lophius litulon was CS-C.

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