作 者：
李萍;史荣久;赵峰;于景华;崔晓阳;胡金贵;张颖
单 位：
中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室;中国科学院沈阳应用生态研究所;内蒙古汗马自然保护区管理局;东北林业大学
关键词：
落叶松;16S rDNA;土壤细菌多样性;功能预测;元素循环
摘 要：
采用土壤细菌16S r DNA高通量测序方法研究了大兴安岭汗马自然保护区落叶松林不同演替阶段的土壤细菌群落结构和功能.结果表明:不同演替阶段落叶松林土壤细菌优势门为变形菌门、酸杆菌门、疣微菌门、拟杆菌门、放线菌门、浮霉菌门和绿弯菌门,随着演替的进行,酸杆菌门相对丰度逐渐增加,绿弯菌门相对丰度逐渐减少,优势门相对丰度在不同演替阶段不同.细菌群落α多样性在不同演替阶段间无显著差异,但其群落结构分别在落叶松幼龄林与中龄林、幼龄林与过熟林、近熟林与成熟林之间存在显著差异.土壤氧化还原电位、土壤p H和有效磷是影响细菌群落结构的主要环境因素,其中土壤氧化还原电位对微生物群落结构影响最大.随着演替的进行,细菌参与的固氮作用、反硝化作用、氨氧化作用、木质素降解作用呈逐渐减弱的趋势,硫酸盐异化还原作用呈先降后升的趋势,碳固定作用呈先升后降的趋势,碱性磷酸酶没有明显的变化规律,影响土壤功能的主要因素有土壤有效磷和氧化还原电位等.
译 名：
Soil bacterial community structure and predicted functions in the larch forest during succession at the Greater Khingan Mountains of Northeast China
作 者：
LI Ping;SHI Rong-jiu;ZHAO Feng;YU Jing-hua;CUI Xiao-yang;HU Jin-gui;ZHANG Ying;Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences;University of Chinese Academy of Sciences;Northeast Forestry University;Inner Mongolia Hanma National Nature Reserve Administration;
单 位：
LI Ping%SHI Rong-jiu%ZHAO Feng%YU Jing-hua%CUI Xiao-yang%HU Jin-gui%ZHANG Ying%Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences%University of Chinese Academy of Sciences%Northeast Forestry University%Inner Mongolia Hanma National Nature Reserve Administration
关键词：
larch;;16S r DNA;;soil bacterial diversity;;function predicting;;element cycle
摘 要：
To reveal soil bacterial community structure and potential functions in larch forest during succession at Greater Khingan Mountains( Hanma National Nature Reserve),16 S r DNA was sequencing by Illumina Miseq. The results showed that the Proteobacteria,Acidobacteria,Verrucomicrobia,Bacteroidetes,Actinobacteria,Planctomycetes and Chloroflexi were the most dominant phyla in soils of larch forests at various successional stages. Along forest succession,Acidobacteria increased,while Chloroflexi decreased. Relative abundance of dominant phyla was different at various successional stages. The α diversity,Chao1,Shannon index and Simpson index of soil bacterial community had no significant difference among five succession stages,while significant differences in soil bacterial community structure were observed between young and medium larch,between young and over mature larch,and between near mature and mature larch. Bacterial community structure was mainly influenced by redox potential,pH and available phosphorus. The redox potential was the most important factor influencing soil bacterial community structure. Along the succession of larch forest,N-fixation,denitrification,ammonia oxidation and lignin breakdown decreased,dissimilatory sulfate reduction had down-up trend,carbon fixation had up-down trend,and alkaline phosphatase had no apparent trend. Bacterial community potential function was mainly influenced by redox potential and available phosphorus.
