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Position: Home > Articles > Genomic DNA Extraction and Optimization of RAPD Analytic Conditions of Aprostocetus prolixus Science of Sericulture 2006,32 (4) 152-155

桑天牛卵啮小蜂基因组DNA的提取及RAPD反应体系的优化

作  者:
袁芳芳;黄大庄;王志刚;王宝辉
单  位:
河北农业大学
关键词:
桑天牛卵啮小蜂;DNA提取;随机扩增多态性DNA
摘  要:
桑天牛卵啮小蜂是抑制桑天牛的有效天敌。为使天敌能大量人工繁殖,从分子生物学角度对单头桑天牛卵啮小蜂基因组DNA的提取方法及RAPD反应条件进行了探索。结果表明,采用改良的SDS方法,提取的DNA质量较高,适宜于RAPD-PCR扩增分析。在25μL反应体系中,RAPD分析的优化反应体系为:1.5mmol/L Mg~(2+)、2μL的dNTPs(2.5mmol/L)、320nmol/L随机引物、80ng模板DNA、0.8U TaqDNA聚合酶。
译  名:
Genomic DNA Extraction and Optimization of RAPD Analytic Conditions of Aprostocetus prolixus
作  者:
YUAN Fang-Fang HUANG Da-Zhuang~* WANG Zhi-Gang WANG Bao-Hui (Hebei Agricultural University,Baoding Hebei 071000,China)
关键词:
Aprostocetus prolixus;;DNA extraction;;RAPD
摘  要:
Aprostocetus prolixus is an important natural enemy for Apriona germari.In order to propagate more Aprostocetus prolixus,we studied the method of genomic DNA extraction and optimization of RAPD ana- lytic conditions.The results showed that high grade genome DNA was obtained by the revised SDS method. The optimal PCR system for RAPD analysis was as follows:1.5mmol/L Mg~(2+),2μLdNTPs(2.5mmol/L),320 nmol/L random primer,80ng DNA template,0.8 U Taq polymerase,in 25μL reaction system.

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