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Position: Home > Articles > HPLC Determination of Vitamin E Content in Highland Barley from Tibetan Plateau FOOD SCIENCE 2009,30 (6) 213-215

青藏地区青稞中VE的高效液相色谱测定

作  者:
张春红;许传梅;董琦;胡凤祖
单  位:
中国科学院西北高原生物研究所
关键词:
高效液相色谱(HPLC);青稞;VE
摘  要:
采用高效液相色谱法对青藏高原的16种不同地区和品种的青稞中δ-VE、γ-VE和α-VE同时进行了含量的测定,为青稞的质量评价和综合利用提供依据。采用SymmetryC18(4.6mm×250mm,5μm)色谱柱;甲醇:水=98:2(V/V)为流动相;检测波长295nm;柱温25℃;流速1ml/min。结果表明,青藏地区的青稞中VE总含量在0.3~0.8mg/100g,其中青海地区青稞中VE的平均总含量比西藏地区低约10%。OR
译  名:
HPLC Determination of Vitamin E Content in Highland Barley from Tibetan Plateau
作  者:
ZHANG Chun-hong1,2,XU Chuan-mei1,2,DONG Qi1,HU Feng-zu1,(1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
关键词:
HPLC;highland barley;VE OR
摘  要:
In order to provide scientific basis for the quantity control and comprehensive utilization of highland barley, δ-vitamin E, γ- vitamin E , α- vitamin E contents in 16 cultivars of highland barley from Qinghai and highland barley samples from different 10 areas of Tibet were determined by HPLC. The chromatographic column, mobile phase, column temperature, flow rate and detection wavelength were Symmetry C18 column (4.6 mm × 250 mm, 5 μm), methanol-water at the volume ratio of 98: 2, 25 ℃, 1 ml/min and 295 nm, respectively. The results indicated that all the collected highland barley in Tibetan plateau contain vitamin E,and the total content is between 0.3 mg/100g and 0.8 mg/100 g, especially the average content of total vitamin E in highland barley from Tibet is higher about 10% than that from Qinghai.

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