作 者：
王世成;王颜红;刘艳辉;崔杰华;张红;齐伟;李林平
单 位：
中国科学院沈阳应用生态研究所;中国绿色食品发展中心
关键词：
氢化物发生-原子荧光;有机硒;无机硒;鸭蛋
摘 要：
建立鸭蛋中有机硒和无机硒的测定方法,采用pH8.6的三羟甲基氨基甲烷-盐酸(Tris-HCl)缓冲溶液提取,硫酸铵沉淀后获得无机硒溶液,采用原子荧光法测定其中的总硒和无机硒,采用差减法计算有机硒的含量。结果表明,在优化的条件下,方法无机硒检出限0.003mg/kg,线性范围2.0～50μg/L,相对标准偏差3.21%,方法的加标回收率为82.8%～91.0%。该方法简便、灵敏,可为鸭蛋中有机硒和无机硒的测定提供了一种完善检测方法。
译 名：
Determination of Inorganic and Organic Selenium in Duck Eggs by Hydride Generation-Atomic Fluorescence Spectrometry
作 者：
WANG Shi-cheng1,WANG Yan-hong1,LIU Yan-hui2,CUI Jie-hua1,ZHANG Hong1,QI Wei1,LI Lin-ping1(1.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China; 2.China Green Food Development Center,Beijing 100081,China)
关键词：
hydride generation-atomic fluorescence spectrometry(HG-AFS);organic selenium;inorganic selenium;duck eggs
摘 要：
A method for the determination of organic and inorganic selenium in duck eggs by hydride generation-atomic fluorescence spectrometry(HG-AFS) was developed.A sample was extracted with the buffer solution of Tris(hydroxymethyl) aminomethane-hydrochloric acid(Tris-HCl) in pH 8.6,deposited with Ammonium sulphate.The total selenium and inorganic selenium content were determined with HG-AFS.The difference in the contents of total selenium and the inorganic selenium represents the content of organic selenium.The detection limit for selenium was 0.003 mg/kg under the optimum condition.The linear range was within 2.0–50 μg/L.The relative standard derivation was less than 3.21%.The recovery was 82.8%–91.0%.The inorganic selenium and organic selenium content were detected respectively from the selenium enriched duck eggs by this method.This method met the determination of organic selenium in duck eggs.
